Time-resolved dual transcriptomics of <i>Pseudomonas aeruginosa</i> biofilm formation in cystic fibrosis.

Asensio NC; Rendón JM; González López JJ; Tizzano SG; Martín Gómez MT; Burgas MT

doi:10.1016/j.bioflm.2025.100301

Time-resolved dual transcriptomics of <i>Pseudomonas aeruginosa</i> biofilm formation in cystic fibrosis.

Asensio NC
, Rendón JM
, González López JJ
, Tizzano SG
, Martín Gómez MT
, Burgas MT

Producció científica: Contribució a revista › Article › Recerca › Avaluat per experts

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Resum

Els biofilms de Pseudomonas aeruginosa causen infeccions greus a les vies respiratòries dels pacients que pateixen fibrosi quística (CF) i són difícils d'eradicar, fins i tot amb teràpia antibiòtica intensiva. L'objectiu principal d'aquest estudi era definir la resposta transcripcional dual associada amb la formació de biofilms de P. aeruginosa en una monocapa epitelial pulmonar polaritzada. Analitzàvem la doble resposta dels epitelis sans i CF després de la infecció amb aïllats de P. aeruginosa d'infeccions agudes i cròniques. Els nostres resultats mostren que les soques de P. aeruginosa aïllades d'infeccions cròniques augmenten específicament l'expressió dels sistemes de secreció tipus I, III i VI per segrestar la resposta de l'hoste. Per contra, les soques associades amb malalties agudes usen transportadors ABC per contrarestar la resposta antimicrobiana. En canvi, un patró d'expressió distintiu en els epitelis CF, incloent un alt grau de secreció de citoquines i queratinització, s'observa àmpliament en infeccions agudes. Els nostres resultats mostren que tant el genoma hoste com patogen contribueixen al resultat de la infecció i es poden usar signatures transcripcionals específiques per a diagnòstics, particularment en pacients CF.

Idioma original	Anglès
Nombre de pàgines	10
Revista	Biofilm
Volum	10
DOIs	https://doi.org/10.1016/j.bioflm.2025.100301
Estat de la publicació	Publicada - 2 de jul. 2025

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10.1016/j.bioflm.2025.100301

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@article{dd3180f180b44e7b8d7335ce81d08627,

title = "Time-resolved dual transcriptomics of Pseudomonas aeruginosa biofilm formation in cystic fibrosis.",

abstract = "Pseudomonas aeruginosa biofilms cause severe infections in the airways of patients suffering from cystic fibrosis (CF) that are difficult to eradicate, even with intensive antibiotic therapy. The main goal of this study was to define the dual transcriptional response associated with the formation of P. aeruginosa biofilms in a polarized lung epithelium monolayer. We analyzed the dual response of healthy and CF epithelium after infection with P. aeruginosa isolates from acute and chronic infections. Our results show that strains of P. aeruginosa isolated from chronic infections specifically increase the expression of secretion systems of type I, III and VI to hijack the host response. Conversely, strains associated with acute illness use ABC transporters to counteract the antimicrobial response. In return, a distinctive expression pattern in the CF epithelium, including a high degree of cytokine secretion and keratinization, is largely observed in acute infections. Our results show that both host and pathogen genomic backgrounds contribute to the outcome of infection and specific transcriptional signatures could be used in the diagnosis, particularly in CF patients.",

author = "Asensio NC and Rend{\'o}n JM and JJ, \{Gonz{\'a}lez L{\'o}pez\} and Tizzano SG and MT, \{Mart{\'i}n G{\'o}mez\} and Burgas MT",

year = "2025",

month = jul,

day = "2",

doi = "10.1016/j.bioflm.2025.100301",

language = "English",

volume = "10",

journal = "Biofilm",

issn = "2590-2075",

publisher = "Elsevier BV",

}

TY - JOUR

T1 - Time-resolved dual transcriptomics of Pseudomonas aeruginosa biofilm formation in cystic fibrosis.

AU - NC, Asensio

AU - JM, Rendón

AU - JJ, González López

AU - SG, Tizzano

AU - MT, Martín Gómez

AU - MT, Burgas

PY - 2025/7/2

Y1 - 2025/7/2

N2 - Pseudomonas aeruginosa biofilms cause severe infections in the airways of patients suffering from cystic fibrosis (CF) that are difficult to eradicate, even with intensive antibiotic therapy. The main goal of this study was to define the dual transcriptional response associated with the formation of P. aeruginosa biofilms in a polarized lung epithelium monolayer. We analyzed the dual response of healthy and CF epithelium after infection with P. aeruginosa isolates from acute and chronic infections. Our results show that strains of P. aeruginosa isolated from chronic infections specifically increase the expression of secretion systems of type I, III and VI to hijack the host response. Conversely, strains associated with acute illness use ABC transporters to counteract the antimicrobial response. In return, a distinctive expression pattern in the CF epithelium, including a high degree of cytokine secretion and keratinization, is largely observed in acute infections. Our results show that both host and pathogen genomic backgrounds contribute to the outcome of infection and specific transcriptional signatures could be used in the diagnosis, particularly in CF patients.

AB - Pseudomonas aeruginosa biofilms cause severe infections in the airways of patients suffering from cystic fibrosis (CF) that are difficult to eradicate, even with intensive antibiotic therapy. The main goal of this study was to define the dual transcriptional response associated with the formation of P. aeruginosa biofilms in a polarized lung epithelium monolayer. We analyzed the dual response of healthy and CF epithelium after infection with P. aeruginosa isolates from acute and chronic infections. Our results show that strains of P. aeruginosa isolated from chronic infections specifically increase the expression of secretion systems of type I, III and VI to hijack the host response. Conversely, strains associated with acute illness use ABC transporters to counteract the antimicrobial response. In return, a distinctive expression pattern in the CF epithelium, including a high degree of cytokine secretion and keratinization, is largely observed in acute infections. Our results show that both host and pathogen genomic backgrounds contribute to the outcome of infection and specific transcriptional signatures could be used in the diagnosis, particularly in CF patients.

UR - https://www.scopus.com/pages/publications/105010317507

UR - https://www.mendeley.com/catalogue/5cd87e9b-f391-39ef-8d81-4b81292a2f80/

U2 - 10.1016/j.bioflm.2025.100301

DO - 10.1016/j.bioflm.2025.100301

M3 - Article

C2 - 40697188

SN - 2590-2075

VL - 10

JO - Biofilm

JF - Biofilm

ER -

Time-resolved dual transcriptomics of <i>Pseudomonas aeruginosa</i> biofilm formation in cystic fibrosis.

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