What the human sperm methylome tells us

Cristina Camprubí; Riccardo Aiese Cigliano; Albert Salas-Huetos; Nicolas Garrido; Joan Blanco

doi:10.2217/epi-2017-0049

What the human sperm methylome tells us

Cristina Camprubí, Riccardo Aiese Cigliano, Albert Salas-Huetos, Nicolas Garrido, Joan Blanco

Producción científica: Contribución a una revista › Artículo › Investigación › revisión exhaustiva

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© 2017 Future Medicine Ltd. Aim: To characterize the sperm methylome in semen samples from 19 donors with proven fertility. Materials & methods: Bisulfite-converted sperm DNA was hybridized on the HumanMethylation450 Infinium BeadChip platform. CpG fluorescence intensities were extracted and converted to β-values. Results: The sperm methylome is highly homogeneous and hypomethylated. Genes with hypomethylated promoters are ontologically associated to biological functions related to spermatogenesis and embryogenesis. Sex chromosomes are the most hypomethylated chromosomes, supporting data that indicated their essential role in spermatogenesis. A total of 94 genes are resistant to demethylation, being strong candidates for transgenerational inheritance. Conclusion: Spermatozoa carry a homogeneous methylation profile that is a footprint of past events (spermatogenesis), is designed to facilitate future events (embryogenesis) and has a possible influence in the adult life (transgenerational effects).

Idioma original	Inglés
Páginas (desde-hasta)	1299-1315
Publicación	Epigenomics
Volumen	9
N.º	10
DOI	https://doi.org/10.2217/epi-2017-0049
Estado	Publicada - 1 oct 2017

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title = "What the human sperm methylome tells us",

abstract = "{\textcopyright} 2017 Future Medicine Ltd. Aim: To characterize the sperm methylome in semen samples from 19 donors with proven fertility. Materials & methods: Bisulfite-converted sperm DNA was hybridized on the HumanMethylation450 Infinium BeadChip platform. CpG fluorescence intensities were extracted and converted to β-values. Results: The sperm methylome is highly homogeneous and hypomethylated. Genes with hypomethylated promoters are ontologically associated to biological functions related to spermatogenesis and embryogenesis. Sex chromosomes are the most hypomethylated chromosomes, supporting data that indicated their essential role in spermatogenesis. A total of 94 genes are resistant to demethylation, being strong candidates for transgenerational inheritance. Conclusion: Spermatozoa carry a homogeneous methylation profile that is a footprint of past events (spermatogenesis), is designed to facilitate future events (embryogenesis) and has a possible influence in the adult life (transgenerational effects).",

keywords = "DNA methylation, embryogenesis, methylome, miRNA, piRNA, sex chromosomes, spermatogenesis, spermatozoa, transgenerational epigenetic inheritance",

author = "Cristina Camprub{\'i} and Cigliano, {Riccardo Aiese} and Albert Salas-Huetos and Nicolas Garrido and Joan Blanco",

year = "2017",

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doi = "10.2217/epi-2017-0049",

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journal = "Epigenomics",

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T1 - What the human sperm methylome tells us

AU - Camprubí, Cristina

AU - Cigliano, Riccardo Aiese

AU - Salas-Huetos, Albert

AU - Garrido, Nicolas

AU - Blanco, Joan

PY - 2017/10/1

Y1 - 2017/10/1

N2 - © 2017 Future Medicine Ltd. Aim: To characterize the sperm methylome in semen samples from 19 donors with proven fertility. Materials & methods: Bisulfite-converted sperm DNA was hybridized on the HumanMethylation450 Infinium BeadChip platform. CpG fluorescence intensities were extracted and converted to β-values. Results: The sperm methylome is highly homogeneous and hypomethylated. Genes with hypomethylated promoters are ontologically associated to biological functions related to spermatogenesis and embryogenesis. Sex chromosomes are the most hypomethylated chromosomes, supporting data that indicated their essential role in spermatogenesis. A total of 94 genes are resistant to demethylation, being strong candidates for transgenerational inheritance. Conclusion: Spermatozoa carry a homogeneous methylation profile that is a footprint of past events (spermatogenesis), is designed to facilitate future events (embryogenesis) and has a possible influence in the adult life (transgenerational effects).

AB - © 2017 Future Medicine Ltd. Aim: To characterize the sperm methylome in semen samples from 19 donors with proven fertility. Materials & methods: Bisulfite-converted sperm DNA was hybridized on the HumanMethylation450 Infinium BeadChip platform. CpG fluorescence intensities were extracted and converted to β-values. Results: The sperm methylome is highly homogeneous and hypomethylated. Genes with hypomethylated promoters are ontologically associated to biological functions related to spermatogenesis and embryogenesis. Sex chromosomes are the most hypomethylated chromosomes, supporting data that indicated their essential role in spermatogenesis. A total of 94 genes are resistant to demethylation, being strong candidates for transgenerational inheritance. Conclusion: Spermatozoa carry a homogeneous methylation profile that is a footprint of past events (spermatogenesis), is designed to facilitate future events (embryogenesis) and has a possible influence in the adult life (transgenerational effects).

KW - DNA methylation

KW - embryogenesis

KW - methylome

KW - miRNA

KW - piRNA

KW - sex chromosomes

KW - spermatogenesis

KW - spermatozoa

KW - transgenerational epigenetic inheritance

U2 - 10.2217/epi-2017-0049

DO - 10.2217/epi-2017-0049

M3 - Article

SN - 1750-1911

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SP - 1299

EP - 1315

JO - Epigenomics

JF - Epigenomics

IS - 10

ER -

What the human sperm methylome tells us

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