Tetracycline resistance transmission in Campylobacter is promoted at temperatures resembling the avian reservoir

Ferran Navarro Risueño, E. Cuevas-Ferrando, Pedro Guirado, Elisenda Miró, Yaidelys Iglesias Torrens, Tyler Scott Alioto, Jessica Gómez-Garrido, Cristina Madrid Xufré, Carlos Balsalobre Parra

Producción científica: Contribución a una revistaArtículoInvestigaciónrevisión exhaustiva

8 Citas (Scopus)
2 Descargas (Pure)

Resumen

Campylobacter is the causal agent of campylobacteriosis in humans, a self-limiting gastroenteritis. Campylobacteriosis is a zoonosis, commonly transmitted from contaminated chicken meat by either direct consumption or cross contamination during food manipulation. Presence of plasmids encoding for resistance to antibiotics such as tetracycline is common among Campylobacter isolates. In this report, we studied the effect of the temperature in the conjugation frequency of several tet(O) carrying plasmids, providing tetracycline resistance to the recipient cells. The conjugation frequency from donor cells carrying three previously characterized plasmids (pCjA13, pCjA9 and pTet) and from two clinical isolates was determined. Two temperatures, 37 and 42 °C, mimicking the conditions encountered by C. jejuni in the human and broiler chicken gastrointestinal tracts, respectively, were assessed. Our results clearly indicate that the conjugation process is promoted at high temperature. Accordingly, the transcriptional expression of some putative conjugative apparatus genes is thermoregulated, being induced at 42 °C. The two plasmids present in the clinical isolates were sequenced and assembled. Both plasmids are highly related among them and to the pTet plasmid. The high identity of the genes putatively involved in the conjugation process among the plasmids is in agreement with the similar behavior regarding the temperature dependency of the conjugative process. This report suggest that conjugation of plasmids carrying antibiotic resistance genes occurs preferentially at temperatures that resemble the gastrointestinal tract of birds, the main reservoir of C. jejuni.

Idioma originalInglés
Número de artículo108652
PublicaciónVeterinary Microbiology
Volumen244
DOI
EstadoPublicada - may 2020

Huella

Profundice en los temas de investigación de 'Tetracycline resistance transmission in Campylobacter is promoted at temperatures resembling the avian reservoir'. En conjunto forman una huella única.

Citar esto