Structure-guided redesign of d-fructose-6-phosphate aldolase from E. coli: Remarkable activity and selectivity towards acceptor substrates by two-point mutation

Mariana Gutierrez; Teodor Parella; Jesús Joglar; Jordi Bujons; Pere Clapés

doi:10.1039/c1cc11069a

Structure-guided redesign of d-fructose-6-phosphate aldolase from E. coli: Remarkable activity and selectivity towards acceptor substrates by two-point mutation

Mariana Gutierrez, Teodor Parella, Jesús Joglar, Jordi Bujons, Pere Clapés

Producción científica: Contribución a una revista › Artículo › Investigación › revisión exhaustiva

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Structure-guided re-design of the acceptor binding site of d-fructose-6-phosphate aldolase from E. coli leads to the construction of FSA A129S/A165G double mutant with an activity between 5- to >900-fold higher than that of wild-type towards N-Cbz-aminoaldehyde derivatives. © 2011 The Royal Society of Chemistry.

Idioma original	Inglés
Páginas (desde-hasta)	5762-5764
Publicación	Chemical Communications
Volumen	47
N.º	20
DOI	https://doi.org/10.1039/c1cc11069a
Estado	Publicada - 28 may 2011

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abstract = "Structure-guided re-design of the acceptor binding site of d-fructose-6-phosphate aldolase from E. coli leads to the construction of FSA A129S/A165G double mutant with an activity between 5- to >900-fold higher than that of wild-type towards N-Cbz-aminoaldehyde derivatives. {\textcopyright} 2011 The Royal Society of Chemistry.",

author = "Mariana Gutierrez and Teodor Parella and Jes{\'u}s Joglar and Jordi Bujons and Pere Clap{\'e}s",

year = "2011",

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Structure-guided redesign of d-fructose-6-phosphate aldolase from E. coli: Remarkable activity and selectivity towards acceptor substrates by two-point mutation. / Gutierrez, Mariana; Parella, Teodor; Joglar, Jesús et al.
En: Chemical Communications, Vol. 47, N.º 20, 28.05.2011, p. 5762-5764.

Producción científica: Contribución a una revista › Artículo › Investigación › revisión exhaustiva

TY - JOUR

T1 - Structure-guided redesign of d-fructose-6-phosphate aldolase from E. coli: Remarkable activity and selectivity towards acceptor substrates by two-point mutation

AU - Gutierrez, Mariana

AU - Parella, Teodor

AU - Joglar, Jesús

AU - Bujons, Jordi

AU - Clapés, Pere

PY - 2011/5/28

Y1 - 2011/5/28

N2 - Structure-guided re-design of the acceptor binding site of d-fructose-6-phosphate aldolase from E. coli leads to the construction of FSA A129S/A165G double mutant with an activity between 5- to >900-fold higher than that of wild-type towards N-Cbz-aminoaldehyde derivatives. © 2011 The Royal Society of Chemistry.

AB - Structure-guided re-design of the acceptor binding site of d-fructose-6-phosphate aldolase from E. coli leads to the construction of FSA A129S/A165G double mutant with an activity between 5- to >900-fold higher than that of wild-type towards N-Cbz-aminoaldehyde derivatives. © 2011 The Royal Society of Chemistry.

U2 - 10.1039/c1cc11069a

DO - 10.1039/c1cc11069a

M3 - Article

SN - 1359-7345

VL - 47

SP - 5762

EP - 5764

JO - Chemical Communications

JF - Chemical Communications

IS - 20

ER -

Structure-guided redesign of d-fructose-6-phosphate aldolase from E. coli: Remarkable activity and selectivity towards acceptor substrates by two-point mutation

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