Reference values for interleukin‐6 in the amniotic fluid of asymptomatic pregnant women

Ester del Barco; Clara Franco‐Jarava; Mireia Vargas; Nerea Maíz; Silvia Arevalo; Maria Ángeles Sánchez; Maria Teresa Avilés; Carlota Rodó; Manel Mendoza; Maria Goya; Manuel Hernández‐González; Elena Carreras

doi:10.1111/aogs.14524

Reference values for interleukin‐6 in the amniotic fluid of asymptomatic pregnant women

Ester del Barco, Clara Franco‐Jarava, Mireia Vargas, Nerea Maíz, Silvia Arevalo, Maria Ángeles Sánchez, Maria Teresa Avilés, Carlota Rodó, Manel Mendoza, Maria Goya, Manuel Hernández‐González, Elena Carreras

Departamento de Pediatría, de Obstetricia y Ginecología y de Medicina Preventiva y Salud Pública

Producción científica: Contribución a una revista › Artículo › Investigación › revisión exhaustiva

5 Citas (Scopus)

Resumen

Introduction
Nowadays, proinflammatory factors are considered to play an important role in the pathophysiology of threatened preterm labor or chorioamnionitis. The aim of this study was to establish the normal reference range for interleukin-6 (IL-6) levels in the amniotic fluid and to identify factors which may alter this value.

Material and methods
Prospective study in a tertiary-level center including asymptomatic pregnant women undergoing amniocentesis for genetic studies from October 2016 to September 2019. IL-6 measurements in amniotic fluid were performed using a fluorescence immunoassay with microfluidic technology (ELLA Proteinsimple, Bio Techne). Maternal history and pregnancy data were also recorded.

Results
This study included 140 pregnant women. Of those, women who underwent termination of pregnancy were excluded. Therefore, a total of 98 pregnancies were included in the final statistical analysis. The mean gestational age was 21.86 weeks (range: 15–38.7) at the time of amniocentesis, and 38.6 weeks (range: 30.9–41.4) at delivery. No cases of chorioamnionitis were reported. The log10 IL-6 values follow a normal distribution (W = 0.990, p = 0.692).

The median, and the 5th, 10th, 90th, and 95th percentiles for IL-6 levels were 573, 105, 130, 1645, and 2260 pg/mL, respectively. The log10 IL-6 values were not affected by gestational age (p = 0.395), maternal age (p = 0.376), body mass index (p = 0.551), ethnicity (p = 0.467), smoking status (p = 0.933), parity (p = 0.557), method of conception (p = 0.322), or diabetes mellitus (p = 0.381).

Conclusions
The log10 IL-6 values follow a normal distribution. IL-6 values are independent of gestational age, maternal age, body mass index, ethnicity, smoking status, parity and method of conception. Our study provides a normal reference range for IL-6 levels in the amniotic fluid that can be used in future studies. We also observed that normal IL-6 values were higher in the amniotic fluid than in serum.

Idioma original	Inglés
Páginas (desde-hasta)	480-485
Número de páginas	6
Publicación	Acta Obstetricia et Gynecologica Scandinavica
Volumen	102
N.º	4
DOI	https://doi.org/10.1111/aogs.14524
Estado	Publicada - 11 mar 2023

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10.1111/aogs.14524Licencia: CC BY-NC

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Barco, E. D., Franco‐Jarava, C., Vargas, M., Maíz, N., Arevalo, S., Sánchez, M. Á., Avilés, M. T., Rodó, C., Mendoza, M., Goya, M., Hernández‐González, M., & Carreras, E. (2023). Reference values for interleukin‐6 in the amniotic fluid of asymptomatic pregnant women. Acta Obstetricia et Gynecologica Scandinavica, 102(4), 480-485. https://doi.org/10.1111/aogs.14524

@article{413334fa3e1f4300a57043692b7cbcf5,

title = "Reference values for interleukin‐6 in the amniotic fluid of asymptomatic pregnant women",

abstract = "Introduction Nowadays, proinflammatory factors are considered to play an important role in the pathophysiology of threatened preterm labor or chorioamnionitis. The aim of this study was to establish the normal reference range for interleukin-6 (IL-6) levels in the amniotic fluid and to identify factors which may alter this value. Material and methods Prospective study in a tertiary-level center including asymptomatic pregnant women undergoing amniocentesis for genetic studies from October 2016 to September 2019. IL-6 measurements in amniotic fluid were performed using a fluorescence immunoassay with microfluidic technology (ELLA Proteinsimple, Bio Techne). Maternal history and pregnancy data were also recorded. Results This study included 140 pregnant women. Of those, women who underwent termination of pregnancy were excluded. Therefore, a total of 98 pregnancies were included in the final statistical analysis. The mean gestational age was 21.86 weeks (range: 15–38.7) at the time of amniocentesis, and 38.6 weeks (range: 30.9–41.4) at delivery. No cases of chorioamnionitis were reported. The log10 IL-6 values follow a normal distribution (W = 0.990, p = 0.692). The median, and the 5th, 10th, 90th, and 95th percentiles for IL-6 levels were 573, 105, 130, 1645, and 2260 pg/mL, respectively. The log10 IL-6 values were not affected by gestational age (p = 0.395), maternal age (p = 0.376), body mass index (p = 0.551), ethnicity (p = 0.467), smoking status (p = 0.933), parity (p = 0.557), method of conception (p = 0.322), or diabetes mellitus (p = 0.381). Conclusions The log10 IL-6 values follow a normal distribution. IL-6 values are independent of gestational age, maternal age, body mass index, ethnicity, smoking status, parity and method of conception. Our study provides a normal reference range for IL-6 levels in the amniotic fluid that can be used in future studies. We also observed that normal IL-6 values were higher in the amniotic fluid than in serum.",

keywords = "Amniocentesis, Amniotic fluid, Chorioamnionitis, Interleukin-6, Preterm labor, Intra-amniotic infection, Intra-amniotic inflammation, Spontaneous preterm delivery",

author = "Barco, \{Ester del\} and Clara Franco‐Jarava and Mireia Vargas and Nerea Ma{\'i}z and Silvia Arevalo and S{\'a}nchez, \{Maria {\'A}ngeles\} and Avil{\'e}s, \{Maria Teresa\} and Carlota Rod{\'o} and Manel Mendoza and Maria Goya and Manuel Hern{\'a}ndez‐Gonz{\'a}lez and Elena Carreras",

note = "Publisher Copyright: {\textcopyright} 2023 The Authors. Acta Obstetricia et Gynecologica Scandinavica published by John Wiley \& Sons Ltd on behalf of Nordic Federation of Societies of Obstetrics and Gynecology (NFOG).",

year = "2023",

month = mar,

day = "11",

doi = "10.1111/aogs.14524",

language = "English",

volume = "102",

pages = "480--485",

journal = "Acta Obstetricia et Gynecologica Scandinavica",

issn = "0001-6349",

number = "4",

}

Barco, ED, Franco‐Jarava, C, Vargas, M, Maíz, N, Arevalo, S, Sánchez, MÁ, Avilés, MT, Rodó, C, Mendoza, M, Goya, M, Hernández‐González, M & Carreras, E 2023, 'Reference values for interleukin‐6 in the amniotic fluid of asymptomatic pregnant women', Acta Obstetricia et Gynecologica Scandinavica, vol. 102, n.º 4, pp. 480-485. https://doi.org/10.1111/aogs.14524

TY - JOUR

T1 - Reference values for interleukin‐6 in the amniotic fluid of asymptomatic pregnant women

AU - Barco, Ester del

AU - Franco‐Jarava, Clara

AU - Vargas, Mireia

AU - Maíz, Nerea

AU - Arevalo, Silvia

AU - Sánchez, Maria Ángeles

AU - Avilés, Maria Teresa

AU - Rodó, Carlota

AU - Mendoza, Manel

AU - Goya, Maria

AU - Hernández‐González, Manuel

AU - Carreras, Elena

N1 - Publisher Copyright: © 2023 The Authors. Acta Obstetricia et Gynecologica Scandinavica published by John Wiley & Sons Ltd on behalf of Nordic Federation of Societies of Obstetrics and Gynecology (NFOG).

PY - 2023/3/11

Y1 - 2023/3/11

N2 - Introduction Nowadays, proinflammatory factors are considered to play an important role in the pathophysiology of threatened preterm labor or chorioamnionitis. The aim of this study was to establish the normal reference range for interleukin-6 (IL-6) levels in the amniotic fluid and to identify factors which may alter this value. Material and methods Prospective study in a tertiary-level center including asymptomatic pregnant women undergoing amniocentesis for genetic studies from October 2016 to September 2019. IL-6 measurements in amniotic fluid were performed using a fluorescence immunoassay with microfluidic technology (ELLA Proteinsimple, Bio Techne). Maternal history and pregnancy data were also recorded. Results This study included 140 pregnant women. Of those, women who underwent termination of pregnancy were excluded. Therefore, a total of 98 pregnancies were included in the final statistical analysis. The mean gestational age was 21.86 weeks (range: 15–38.7) at the time of amniocentesis, and 38.6 weeks (range: 30.9–41.4) at delivery. No cases of chorioamnionitis were reported. The log10 IL-6 values follow a normal distribution (W = 0.990, p = 0.692). The median, and the 5th, 10th, 90th, and 95th percentiles for IL-6 levels were 573, 105, 130, 1645, and 2260 pg/mL, respectively. The log10 IL-6 values were not affected by gestational age (p = 0.395), maternal age (p = 0.376), body mass index (p = 0.551), ethnicity (p = 0.467), smoking status (p = 0.933), parity (p = 0.557), method of conception (p = 0.322), or diabetes mellitus (p = 0.381). Conclusions The log10 IL-6 values follow a normal distribution. IL-6 values are independent of gestational age, maternal age, body mass index, ethnicity, smoking status, parity and method of conception. Our study provides a normal reference range for IL-6 levels in the amniotic fluid that can be used in future studies. We also observed that normal IL-6 values were higher in the amniotic fluid than in serum.

AB - Introduction Nowadays, proinflammatory factors are considered to play an important role in the pathophysiology of threatened preterm labor or chorioamnionitis. The aim of this study was to establish the normal reference range for interleukin-6 (IL-6) levels in the amniotic fluid and to identify factors which may alter this value. Material and methods Prospective study in a tertiary-level center including asymptomatic pregnant women undergoing amniocentesis for genetic studies from October 2016 to September 2019. IL-6 measurements in amniotic fluid were performed using a fluorescence immunoassay with microfluidic technology (ELLA Proteinsimple, Bio Techne). Maternal history and pregnancy data were also recorded. Results This study included 140 pregnant women. Of those, women who underwent termination of pregnancy were excluded. Therefore, a total of 98 pregnancies were included in the final statistical analysis. The mean gestational age was 21.86 weeks (range: 15–38.7) at the time of amniocentesis, and 38.6 weeks (range: 30.9–41.4) at delivery. No cases of chorioamnionitis were reported. The log10 IL-6 values follow a normal distribution (W = 0.990, p = 0.692). The median, and the 5th, 10th, 90th, and 95th percentiles for IL-6 levels were 573, 105, 130, 1645, and 2260 pg/mL, respectively. The log10 IL-6 values were not affected by gestational age (p = 0.395), maternal age (p = 0.376), body mass index (p = 0.551), ethnicity (p = 0.467), smoking status (p = 0.933), parity (p = 0.557), method of conception (p = 0.322), or diabetes mellitus (p = 0.381). Conclusions The log10 IL-6 values follow a normal distribution. IL-6 values are independent of gestational age, maternal age, body mass index, ethnicity, smoking status, parity and method of conception. Our study provides a normal reference range for IL-6 levels in the amniotic fluid that can be used in future studies. We also observed that normal IL-6 values were higher in the amniotic fluid than in serum.

KW - Amniocentesis

KW - Amniotic fluid

KW - Chorioamnionitis

KW - Interleukin-6

KW - Preterm labor

KW - Intra-amniotic infection

KW - Intra-amniotic inflammation

KW - Spontaneous preterm delivery

UR - https://www.scopus.com/pages/publications/85150211555

U2 - 10.1111/aogs.14524

DO - 10.1111/aogs.14524

M3 - Article

C2 - 36906815

SN - 0001-6349

VL - 102

SP - 480

EP - 485

JO - Acta Obstetricia et Gynecologica Scandinavica

JF - Acta Obstetricia et Gynecologica Scandinavica

IS - 4

ER -

Reference values for interleukin‐6 in the amniotic fluid of asymptomatic pregnant women

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