Protein kinase CK2 associates to lipid rafts and its pharmacological inhibition enhances neurotransmitter release

Carles Gil; Anton Falqués; Eduard Sarró; Roger Cubí; Juan Blasi; José Aguilera; Emilio Itarte

doi:10.1016/j.febslet.2010.12.029

Protein kinase CK2 associates to lipid rafts and its pharmacological inhibition enhances neurotransmitter release

Carles Gil, Anton Falqués, Eduard Sarró, Roger Cubí, Juan Blasi, José Aguilera, Emilio Itarte

Producción científica: Contribución a una revista › Artículo › Investigación › revisión exhaustiva

12 Citas (Scopus)

Resumen

In the present work we report the presence of protein kinase CK2 in lipid raft preparations from rat brain synaptosomes, obtained after detergent extraction and subsequent isolation of detergent-resistant membranes using sucrose gradient ultracentrifugation. Moreover, the phosphorylation of syntaxin-1 at Ser14, a specific CK2 target, has been detected in lipid rafts, as assessed by a phospho-specific antibody. Treatment with DMAT, a specific CK2 inhibitor, results in a decrease of syntaxin-1 Ser14 phosphorylation in lipid rafts, while the glutamate release from synaptosomes is enhanced. In conclusion, CK2 might control neurotransmitter release by acting on SNARE proteins attached to cholesterol-enriched microdomains. © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Idioma original	Inglés
Páginas (desde-hasta)	414-420
Publicación	FEBS Letters
Volumen	585
N.º	2
DOI	https://doi.org/10.1016/j.febslet.2010.12.029
Estado	Publicada - 21 ene 2011

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title = "Protein kinase CK2 associates to lipid rafts and its pharmacological inhibition enhances neurotransmitter release",

abstract = "In the present work we report the presence of protein kinase CK2 in lipid raft preparations from rat brain synaptosomes, obtained after detergent extraction and subsequent isolation of detergent-resistant membranes using sucrose gradient ultracentrifugation. Moreover, the phosphorylation of syntaxin-1 at Ser14, a specific CK2 target, has been detected in lipid rafts, as assessed by a phospho-specific antibody. Treatment with DMAT, a specific CK2 inhibitor, results in a decrease of syntaxin-1 Ser14 phosphorylation in lipid rafts, while the glutamate release from synaptosomes is enhanced. In conclusion, CK2 might control neurotransmitter release by acting on SNARE proteins attached to cholesterol-enriched microdomains. {\textcopyright} 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.",

keywords = "Cholesterol, Glutamate release, Lipid raft, Protein kinase CK2, Synaptosome, Syntaxin",

author = "Carles Gil and Anton Falqu{\'e}s and Eduard Sarr{\'o} and Roger Cub{\'i} and Juan Blasi and Jos{\'e} Aguilera and Emilio Itarte",

year = "2011",

month = jan,

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doi = "10.1016/j.febslet.2010.12.029",

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TY - JOUR

T1 - Protein kinase CK2 associates to lipid rafts and its pharmacological inhibition enhances neurotransmitter release

AU - Gil, Carles

AU - Falqués, Anton

AU - Sarró, Eduard

AU - Cubí, Roger

AU - Blasi, Juan

AU - Aguilera, José

AU - Itarte, Emilio

PY - 2011/1/21

Y1 - 2011/1/21

N2 - In the present work we report the presence of protein kinase CK2 in lipid raft preparations from rat brain synaptosomes, obtained after detergent extraction and subsequent isolation of detergent-resistant membranes using sucrose gradient ultracentrifugation. Moreover, the phosphorylation of syntaxin-1 at Ser14, a specific CK2 target, has been detected in lipid rafts, as assessed by a phospho-specific antibody. Treatment with DMAT, a specific CK2 inhibitor, results in a decrease of syntaxin-1 Ser14 phosphorylation in lipid rafts, while the glutamate release from synaptosomes is enhanced. In conclusion, CK2 might control neurotransmitter release by acting on SNARE proteins attached to cholesterol-enriched microdomains. © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

AB - In the present work we report the presence of protein kinase CK2 in lipid raft preparations from rat brain synaptosomes, obtained after detergent extraction and subsequent isolation of detergent-resistant membranes using sucrose gradient ultracentrifugation. Moreover, the phosphorylation of syntaxin-1 at Ser14, a specific CK2 target, has been detected in lipid rafts, as assessed by a phospho-specific antibody. Treatment with DMAT, a specific CK2 inhibitor, results in a decrease of syntaxin-1 Ser14 phosphorylation in lipid rafts, while the glutamate release from synaptosomes is enhanced. In conclusion, CK2 might control neurotransmitter release by acting on SNARE proteins attached to cholesterol-enriched microdomains. © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

KW - Cholesterol

KW - Glutamate release

KW - Lipid raft

KW - Protein kinase CK2

KW - Synaptosome

KW - Syntaxin

U2 - 10.1016/j.febslet.2010.12.029

DO - 10.1016/j.febslet.2010.12.029

M3 - Article

SN - 0014-5793

VL - 585

SP - 414

EP - 420

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Protein kinase CK2 associates to lipid rafts and its pharmacological inhibition enhances neurotransmitter release

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