Production of functional inclusion bodies in endotoxin-free Escherichia coli

Fabián Rueda, Olivia Cano-Garrido, Uwe Mamat, Kathleen Wilke, Joaquin Seras-Franzoso, Elena García-Fruitós, Antonio Villaverde

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43 Citas (Scopus)

Resumen

© 2014, Springer-Verlag Berlin Heidelberg. Escherichia coli is the workhorse for gene cloning and production of soluble recombinant proteins in both biotechnological and biomedical industries. The bacterium is also a good producer of several classes of protein-based self-assembling materials such as inclusion bodies (IBs). Apart from being a relatively pure source of protein for in vitro refolding, IBs are under exploration as functional, protein-releasing materials in regenerative medicine and protein replacement therapies. Endotoxin removal is a critical step for downstream applications of therapeutic proteins. The same holds true for IBs as they are often highly contaminated with cell-wall components of the host cells. Here, we have investigated the production of IBs in a recently developed endotoxin-free E. coli strain. The characterization of IBs revealed this mutant as a very useful cell factory for the production of functional endotoxin-free IBs that are suitable for the use at biological interfaces without inducing endotoxic responses in human immune cells.
Idioma originalInglés
Páginas (desde-hasta)9229-9238
PublicaciónApplied Microbiology and Biotechnology
Volumen98
DOI
EstadoPublicada - 1 ene 2014

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