TY - JOUR
T1 - Production of functional inclusion bodies in endotoxin-free Escherichia coli
AU - Rueda, Fabián
AU - Cano-Garrido, Olivia
AU - Mamat, Uwe
AU - Wilke, Kathleen
AU - Seras-Franzoso, Joaquin
AU - García-Fruitós, Elena
AU - Villaverde, Antonio
PY - 2014/1/1
Y1 - 2014/1/1
N2 - © 2014, Springer-Verlag Berlin Heidelberg. Escherichia coli is the workhorse for gene cloning and production of soluble recombinant proteins in both biotechnological and biomedical industries. The bacterium is also a good producer of several classes of protein-based self-assembling materials such as inclusion bodies (IBs). Apart from being a relatively pure source of protein for in vitro refolding, IBs are under exploration as functional, protein-releasing materials in regenerative medicine and protein replacement therapies. Endotoxin removal is a critical step for downstream applications of therapeutic proteins. The same holds true for IBs as they are often highly contaminated with cell-wall components of the host cells. Here, we have investigated the production of IBs in a recently developed endotoxin-free E. coli strain. The characterization of IBs revealed this mutant as a very useful cell factory for the production of functional endotoxin-free IBs that are suitable for the use at biological interfaces without inducing endotoxic responses in human immune cells.
AB - © 2014, Springer-Verlag Berlin Heidelberg. Escherichia coli is the workhorse for gene cloning and production of soluble recombinant proteins in both biotechnological and biomedical industries. The bacterium is also a good producer of several classes of protein-based self-assembling materials such as inclusion bodies (IBs). Apart from being a relatively pure source of protein for in vitro refolding, IBs are under exploration as functional, protein-releasing materials in regenerative medicine and protein replacement therapies. Endotoxin removal is a critical step for downstream applications of therapeutic proteins. The same holds true for IBs as they are often highly contaminated with cell-wall components of the host cells. Here, we have investigated the production of IBs in a recently developed endotoxin-free E. coli strain. The characterization of IBs revealed this mutant as a very useful cell factory for the production of functional endotoxin-free IBs that are suitable for the use at biological interfaces without inducing endotoxic responses in human immune cells.
KW - E. coli
KW - Endotoxin
KW - Inclusion bodies
KW - LPS
KW - LPS-free
KW - Recombinant protein
U2 - 10.1007/s00253-014-6008-9
DO - 10.1007/s00253-014-6008-9
M3 - Article
SN - 0175-7598
VL - 98
SP - 9229
EP - 9238
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
ER -