TY - JOUR
T1 - Polyphenols and IUGR pregnancies :
T2 - effects of the antioxidant hydroxytyrosol on the hippocampus proteome in a porcine model
AU - Yeste, Natalia
AU - Pérez-Valle, Jorge
AU - Vázquez-Gómez, Marta
AU - García-Contreras, Consolación
AU - Gonzalez-Bulnes, Antonio
AU - Bassols Teixidó, Anna Maria
PY - 2022
Y1 - 2022
N2 - Supplementation of a mother's diet with antioxidants such as hydroxytyrosol (HTX) has been proposed to ameliorate the adverse phenotypes of foetuses affected by intrauterine growth restriction (IUGR). Our previous studies showed, in a porcine model of IUGR, an effect of maternal HTX supplementation on the neurotransmitter profile of several brain areas and the morphology of the hippocampus in 100 days old foetuses. The present study analyzed the impact of maternal HTX supplementation on the hippocampus proteome at this foetal age by TMT10plex labelling. Eleven differentially abundant proteins were identified by comparing both conditions, and eight of them downregulated and three upregulated in the HTX-treated group. The downregulated proteins were mainly involved in protein synthesis and RNA metabolism and may explain the differences in neuron differentiation in the HTX-treated group. The upregulated proteins were related to cell detoxification and could represent a potential mechanism to explain the neuroprotective effect of HTX.
AB - Supplementation of a mother's diet with antioxidants such as hydroxytyrosol (HTX) has been proposed to ameliorate the adverse phenotypes of foetuses affected by intrauterine growth restriction (IUGR). Our previous studies showed, in a porcine model of IUGR, an effect of maternal HTX supplementation on the neurotransmitter profile of several brain areas and the morphology of the hippocampus in 100 days old foetuses. The present study analyzed the impact of maternal HTX supplementation on the hippocampus proteome at this foetal age by TMT10plex labelling. Eleven differentially abundant proteins were identified by comparing both conditions, and eight of them downregulated and three upregulated in the HTX-treated group. The downregulated proteins were mainly involved in protein synthesis and RNA metabolism and may explain the differences in neuron differentiation in the HTX-treated group. The upregulated proteins were related to cell detoxification and could represent a potential mechanism to explain the neuroprotective effect of HTX.
KW - Hydroxytyrosol
KW - Hippocampus
KW - Intrauterine growth restriction
KW - Brain
KW - Pig
KW - Proteome
KW - TMT labelling
U2 - 10.3390/antiox11061135
DO - 10.3390/antiox11061135
M3 - Article
C2 - 35740029
SN - 2076-3921
VL - 11
JO - Antioxidants
JF - Antioxidants
IS - 6
ER -