Photoswitchable dynasore analogs to control endocytosis with light

Núria Camarero, Ana Trapero, Ariadna Pérez-Jiménez, Eric Macia, Alexandre Gomila-Juaneda, Andrés Martín-Quirós, Laura Nevola, Artur Llobet, Amadeu Llebaria, Jordi Hernando, Ernest Giralt, Pau Gorostiza*

*Autor correspondiente de este trabajo

Producción científica: Contribución a una revistaArtículoInvestigaciónrevisión exhaustiva

2 Citas (Scopus)

Resumen

The spatiotemporal control of cellular dynamic processes has great fundamental interest but lacks versatile molecular tools. Dynamin is a key protein in endocytosis and an appealing target to manipulate cell trafficking using patterns of light. We have developed the first photoswitchable small-molecule inhibitors of endocytosis (dynazos), by a stepwise design of the photochromic and pharmacological properties of dynasore, a dynamin inhibitor. We have characterized their photochromism with UV-visible and transient absorption spectroscopy and their biological activity using fluorescence microscopies and flow cytometry. Dynazos are water-soluble, cell permeable, and photostable, and enable fast, single-wavelength photoswitchable inhibition of clathrin-mediated endocytosis at micromolar concentration.

Idioma originalInglés
Páginas (desde-hasta)8981-8988
Número de páginas8
PublicaciónChemical Science
Volumen11
N.º33
DOI
EstadoPublicada - 7 sept 2020

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