Northern analysis of highly folded goat α<inf>s1</inf> Casein mRNA

Marta Jansà Pérez; Christine Leroux; Armand Sànchez Bonastre; Patrice Martin

doi:10.1016/S1050-3862(96)80015-5

Northern analysis of highly folded goat α<inf>s1</inf> Casein mRNA

Marta Jansà Pérez, Christine Leroux, Armand Sànchez Bonastre, Patrice Martin

Producción científica: Contribución a una revista › Artículo › Investigación › revisión exhaustiva

Resumen

Northern blotting using glyoxal to denature a highly folded mRNA, such as goat αs1-Casein E, can lead to the detection of multiple incompletely denatured forms. Formaldehyde appears to be the most suitable agent for Northern blotting due to its effective denaturing capacity and lower toxicity than methylmercuric hydroxide.

Idioma original	Inglés
Páginas (desde-hasta)	143-145
Publicación	Genetic analysis : biomolecular engineering
Volumen	12
N.º	3-4
DOI	https://doi.org/10.1016/S1050-3862(96)80015-5
Estado	Publicada - 1 ene 1996

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title = "Northern analysis of highly folded goat αs1 Casein mRNA",

abstract = "Northern blotting using glyoxal to denature a highly folded mRNA, such as goat αs1-Casein E, can lead to the detection of multiple incompletely denatured forms. Formaldehyde appears to be the most suitable agent for Northern blotting due to its effective denaturing capacity and lower toxicity than methylmercuric hydroxide.",

keywords = "Denaturing agents, Ethidium bromide staining, Goat α -casein E s1, Northern blotting",

author = "\{Jans{\`a} P{\'e}rez\}, Marta and Christine Leroux and \{S{\`a}nchez Bonastre\}, Armand and Patrice Martin",

year = "1996",

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doi = "10.1016/S1050-3862(96)80015-5",

language = "English",

volume = "12",

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T1 - Northern analysis of highly folded goat αs1 Casein mRNA

AU - Jansà Pérez, Marta

AU - Leroux, Christine

AU - Sànchez Bonastre, Armand

AU - Martin, Patrice

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Northern blotting using glyoxal to denature a highly folded mRNA, such as goat αs1-Casein E, can lead to the detection of multiple incompletely denatured forms. Formaldehyde appears to be the most suitable agent for Northern blotting due to its effective denaturing capacity and lower toxicity than methylmercuric hydroxide.

AB - Northern blotting using glyoxal to denature a highly folded mRNA, such as goat αs1-Casein E, can lead to the detection of multiple incompletely denatured forms. Formaldehyde appears to be the most suitable agent for Northern blotting due to its effective denaturing capacity and lower toxicity than methylmercuric hydroxide.

KW - Denaturing agents

KW - Ethidium bromide staining

KW - Goat α -casein E s1

KW - Northern blotting

UR - https://www.scopus.com/pages/publications/0347934876

U2 - 10.1016/S1050-3862(96)80015-5

DO - 10.1016/S1050-3862(96)80015-5

M3 - Article

SN - 1050-3862

VL - 12

SP - 143

EP - 145

JO - Genetic analysis : biomolecular engineering

JF - Genetic analysis : biomolecular engineering

IS - 3-4

ER -

Northern analysis of highly folded goat α<inf>s1</inf> Casein mRNA

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