Induction of hydroxyl radical production in Trametes versicolor to degrade recalcitrant chlorinated hydrocarbons

Extracellular hydroxyl radical ({radical dot}OH) production via quinone redox cycling in Trametes versicolor, grown in a chemically defined medium, was investigated to degrade trichloroethylene (TCE), perchloroethylene (PCE), 1,2,4- and 1,3,5-trichlorobenzene (TCB). The activity of the enzymes catalyzing the quinone redox cycle, quinone reductase and laccase, as well as the rate of {radical dot}OH production, estimated as the formation of thiobarbituric acid reactive substances (TBARS) from 2-deoxyribose, increased rapidly during the first 2-3 days and then remained at relatively constant levels. Under quinone redox cycling conditions, TCE degradation was concomitant to TBARS production and chloride release, reaching a plateau after 6 h of incubation. Similar results were obtained in PCE, 1,2,4- and 1,3,5-TCB time course degradation experiments. The mole balance of chloride release and 1,2,4-TCB and TCE degraded suggests that these chemicals were almost completely dechlorinated. Experiments using [13C]-TCE confirmed unequivocal transformation of TCE to 13CO2. These results are of particular interest because PCE and 1,3,5-TCB degradation in aerobic conditions has been rarely reported to date in bacterial or fungal systems. © 2009 Elsevier Ltd. All rights reserved.