Increased glucose transport in ras-transformed fibroblasts: A possible role for N-glycosylation of GLUT1

Rafael Onetti, Josep Baulida, Anna Bassols

Producción científica: Contribución a una revistaArtículoInvestigaciónrevisión exhaustiva

28 Citas (Scopus)

Resumen

2-Deoxyglucose uptake was enhanced in ts371 KiMuSV-NRK cells when growing at the permissive temperature to allow the expression of a transforming p21 ras protein. This change is due to a decrease in the K(m) by approximately 2.5-fold without affecting the V(max) of the transporter. The amount of the GLUT1 glucose transporter dit not increase as deduced from immunoblot experiments on total membranes. Nevertheless, ras-transformed GLUT1 displays a higher molecular mass due to an increased N-glycosylation of the protein. Experiments made in tunicamycin-treated cells indicates that a higher glycosylation is responsible for the increase in 2-deoxyglucose uptake in ras-transformed cells.
Idioma originalInglés
Páginas (desde-hasta)267-270
PublicaciónFEBS Letters
Volumen407
DOI
EstadoPublicada - 5 may 1997

Huella

Profundice en los temas de investigación de 'Increased glucose transport in ras-transformed fibroblasts: A possible role for N-glycosylation of GLUT1'. En conjunto forman una huella única.

Citar esto