Incomplete transcriptional dosage compensation of chicken and platypus sex chromosomes is balanced by post-transcriptional compensation

Nicholas Lister; Ashley M. Milton; Hardip R. Patel; Shafagh A. Waters; Benjamin J. Hanrahan; Kim L. McIntyre; Alexandra M. Livernois; William B. Horspool; Lee Kian Wee; Alessa R. Ringel; Stefan Mundlos; Michael I. Robson; Linda Shearwin-Whyatt; Frank Grutzner; Jennifer A. Marshall Graves; Aurora Ruiz-Herrera Moreno; Paul D. Waters

doi:10.1073/pnas.2322360121

Incomplete transcriptional dosage compensation of chicken and platypus sex chromosomes is balanced by post-transcriptional compensation

Nicholas Lister, Ashley M. Milton, Hardip R. Patel, Shafagh A. Waters, Benjamin J. Hanrahan, Kim L. McIntyre, Alexandra M. Livernois, William B. Horspool, Lee Kian Wee, Alessa R. Ringel, Stefan Mundlos, Michael I. Robson, Linda Shearwin-Whyatt, Frank Grutzner, Jennifer A. Marshall Graves, Aurora Ruiz-Herrera Moreno, Paul D. Waters

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Resumen

Heteromorphic sex chromosomes (XY or ZW) present problems of gene dosage imbalance between sexes and with autosomes. A need for dosage compensation has long been thought to be critical in vertebrates. However, this was questioned by findings of unequal mRNA abundance measurements in monotreme mammals and birds. Here, we demonstrate unbalanced mRNA levels of X genes in platypus males and females and a correlation with differential loading of histone modifications. We also observed unbalanced transcripts of Z genes in chicken. Surprisingly, however, we found that protein abundance ratios were 1:1 between the sexes in both species, indicating a post-transcriptional layer of dosage compensation. We conclude that sex chromosome output is maintained in chicken and platypus (and perhaps many other non therian vertebrates) via a combination of transcriptional and post-transcriptional control, consistent with a critical importance of sex chromosome dosage compensation.

Idioma original	Inglés
Número de artículo	e2322360121
Publicación	Proceedings of the National Academy of Sciences of the United States of America
Volumen	121
N.º	32
DOI	https://doi.org/10.1073/pnas.2322360121
Estado	Publicada - 6 ago 2024

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EXPLORANDO LA DINAMICA DE LA ARQUITECTURA GENOMICA Y LA FUNCION GENICA EN LOS VERTEBRADOS
Marin Garcia, C. (Colaborador/a), Ruiz Herrera Moreno, A. M. (Colaborador/a), Ruiz Herrera Moreno, A. M. (Principal Investigator), Deakin, J. (Colaborador/a), Gonzalez Rodelas, L. (Colaborador/a), WATERS, P. (Colaborador/a), Marín Gual, L. (Colaborador/a), Sanseverino, W. (Colaborador/a), Farré Belmonte, M. (Colaborador/a), Pujol i Infantes, G. (Colaborador/a), ROBINSON, T. (Colaborador/a) & Garcia Haro, F. (Colaborador/a)
1/09/21 → 31/08/25
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Lister, N., Milton, A. M., Patel, H. R., Waters, S. A., Hanrahan, B. J., McIntyre, K. L., Livernois, A. M., Horspool, W. B., Wee, L. K., Ringel, A. R., Mundlos, S., Robson, M. I., Shearwin-Whyatt, L., Grutzner, F., Marshall Graves, J. A., Ruiz-Herrera Moreno, A., & Waters, P. D. (2024). Incomplete transcriptional dosage compensation of chicken and platypus sex chromosomes is balanced by post-transcriptional compensation. Proceedings of the National Academy of Sciences of the United States of America, 121(32), Artículo e2322360121. https://doi.org/10.1073/pnas.2322360121

@article{9448827158764acfa8233338fcf50e63,

title = "Incomplete transcriptional dosage compensation of chicken and platypus sex chromosomes is balanced by post-transcriptional compensation",

abstract = "Heteromorphic sex chromosomes (XY or ZW) present problems of gene dosage imbalance between sexes and with autosomes. A need for dosage compensation has long been thought to be critical in vertebrates. However, this was questioned by findings of unequal mRNA abundance measurements in monotreme mammals and birds. Here, we demonstrate unbalanced mRNA levels of X genes in platypus males and females and a correlation with differential loading of histone modifications. We also observed unbalanced transcripts of Z genes in chicken. Surprisingly, however, we found that protein abundance ratios were 1:1 between the sexes in both species, indicating a post-transcriptional layer of dosage compensation. We conclude that sex chromosome output is maintained in chicken and platypus (and perhaps many other non therian vertebrates) via a combination of transcriptional and post-transcriptional control, consistent with a critical importance of sex chromosome dosage compensation.",

keywords = "dosage compensation, epigenetic, post-transcriptional control, sex chromosome",

author = "Nicholas Lister and Milton, {Ashley M.} and Patel, {Hardip R.} and Waters, {Shafagh A.} and Hanrahan, {Benjamin J.} and McIntyre, {Kim L.} and Livernois, {Alexandra M.} and Horspool, {William B.} and Wee, {Lee Kian} and Ringel, {Alessa R.} and Stefan Mundlos and Robson, {Michael I.} and Linda Shearwin-Whyatt and Frank Grutzner and {Marshall Graves}, {Jennifer A.} and {Ruiz-Herrera Moreno}, Aurora and Waters, {Paul D.}",

note = "Publisher Copyright: Copyright {\textcopyright} 2024 the Author(s)",

year = "2024",

month = aug,

day = "6",

doi = "10.1073/pnas.2322360121",

language = "English",

volume = "121",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

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number = "32",

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Lister, N, Milton, AM, Patel, HR, Waters, SA, Hanrahan, BJ, McIntyre, KL, Livernois, AM, Horspool, WB, Wee, LK, Ringel, AR, Mundlos, S, Robson, MI, Shearwin-Whyatt, L, Grutzner, F, Marshall Graves, JA, Ruiz-Herrera Moreno, A & Waters, PD 2024, 'Incomplete transcriptional dosage compensation of chicken and platypus sex chromosomes is balanced by post-transcriptional compensation', Proceedings of the National Academy of Sciences of the United States of America, vol. 121, n.º 32, e2322360121. https://doi.org/10.1073/pnas.2322360121

Incomplete transcriptional dosage compensation of chicken and platypus sex chromosomes is balanced by post-transcriptional compensation. / Lister, Nicholas; Milton, Ashley M.; Patel, Hardip R. et al.
En: Proceedings of the National Academy of Sciences of the United States of America, Vol. 121, N.º 32, e2322360121, 06.08.2024.

Producción científica: Contribución a una revista › Artículo › Investigación › revisión exhaustiva

TY - JOUR

T1 - Incomplete transcriptional dosage compensation of chicken and platypus sex chromosomes is balanced by post-transcriptional compensation

AU - Lister, Nicholas

AU - Milton, Ashley M.

AU - Patel, Hardip R.

AU - Waters, Shafagh A.

AU - Hanrahan, Benjamin J.

AU - McIntyre, Kim L.

AU - Livernois, Alexandra M.

AU - Horspool, William B.

AU - Wee, Lee Kian

AU - Ringel, Alessa R.

AU - Mundlos, Stefan

AU - Robson, Michael I.

AU - Shearwin-Whyatt, Linda

AU - Grutzner, Frank

AU - Marshall Graves, Jennifer A.

AU - Ruiz-Herrera Moreno, Aurora

AU - Waters, Paul D.

PY - 2024/8/6

Y1 - 2024/8/6

N2 - Heteromorphic sex chromosomes (XY or ZW) present problems of gene dosage imbalance between sexes and with autosomes. A need for dosage compensation has long been thought to be critical in vertebrates. However, this was questioned by findings of unequal mRNA abundance measurements in monotreme mammals and birds. Here, we demonstrate unbalanced mRNA levels of X genes in platypus males and females and a correlation with differential loading of histone modifications. We also observed unbalanced transcripts of Z genes in chicken. Surprisingly, however, we found that protein abundance ratios were 1:1 between the sexes in both species, indicating a post-transcriptional layer of dosage compensation. We conclude that sex chromosome output is maintained in chicken and platypus (and perhaps many other non therian vertebrates) via a combination of transcriptional and post-transcriptional control, consistent with a critical importance of sex chromosome dosage compensation.

AB - Heteromorphic sex chromosomes (XY or ZW) present problems of gene dosage imbalance between sexes and with autosomes. A need for dosage compensation has long been thought to be critical in vertebrates. However, this was questioned by findings of unequal mRNA abundance measurements in monotreme mammals and birds. Here, we demonstrate unbalanced mRNA levels of X genes in platypus males and females and a correlation with differential loading of histone modifications. We also observed unbalanced transcripts of Z genes in chicken. Surprisingly, however, we found that protein abundance ratios were 1:1 between the sexes in both species, indicating a post-transcriptional layer of dosage compensation. We conclude that sex chromosome output is maintained in chicken and platypus (and perhaps many other non therian vertebrates) via a combination of transcriptional and post-transcriptional control, consistent with a critical importance of sex chromosome dosage compensation.

KW - dosage compensation

KW - epigenetic

KW - post-transcriptional control

KW - sex chromosome

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UR - https://www.mendeley.com/catalogue/cfa70628-a2c6-3377-a614-18d57a9a7ccd/

U2 - 10.1073/pnas.2322360121

DO - 10.1073/pnas.2322360121

M3 - Article

C2 - 39074288

SN - 1091-6490

VL - 121

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 32

M1 - e2322360121

ER -

Incomplete transcriptional dosage compensation of chicken and platypus sex chromosomes is balanced by post-transcriptional compensation

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