In vitro cell-transforming potential of secondary polyethylene terephthalate and polylactic acid nanoplastics

Josefa Domenech, Aliro Villacorta, Juan Francisco Ferrer, Raquel Llorens-Chiralt, Ricard Marcos, Alba Hernández, Julia Catalán

Producción científica: Contribución a una revistaArtículoInvestigaciónrevisión exhaustiva


Continuous exposure to plastic pollutants may have serious consequences on human health. However, most toxicity assessments focus on non-environmentally relevant particles and rarely investigate long-term effects such as cancer induction. The present study assessed the carcinogenic potential of two secondary nanoplastics: polyethylene terephthalate (PET) particles generated from plastic bottles, and a biodegradable polylactic acid material, as respective examples of environmentally existing particles and new bioplastics. Pristine polystyrene nanoplastics were also included for comparison. A broad concentration range (6.25-200 μg/mL) of each nanoplastic was tested in both the initiation and promotion conditions of the regulatory assessment-accepted in vitro Bhas 42 cell transformation assay. Parallel cultures allowed confirmation of the efficient cellular internalisation of the three nanoplastics. Cell growth was enhanced by polystyrene in the initiation assay, and by PET in both conditions. Moreover, the number of transformed foci was significantly increased only by the highest PET concentration in the promotion assay, which also showed dose-dependency, indicating that nano PET can act as a non-genotoxic tumour promotor. Together, these findings support the carcinogenic risk assessment of nanoplastics and raise concerns regarding whether real-life co-exposure of PET nanoplastics and other environmental pollutants may result in synergistic transformation capacities.

Idioma originalInglés
Número de artículo134030
Páginas (desde-hasta)134030
PublicaciónJournal of Hazardous Materials
Fecha en línea anticipada13 mar 2024
EstadoPublicada - 5 may 2024


Profundice en los temas de investigación de 'In vitro cell-transforming potential of secondary polyethylene terephthalate and polylactic acid nanoplastics'. En conjunto forman una huella única.

Citar esto