Improved non-radioactive Northern blot protocol for detecting low abundance mRNAs from mammalian tissues

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Resumen

A rapid and sensitive non-radioactive Northern blot protocol is described which has been optimised in several critical steps. This is based on a formaldehyde-denaturing agarose gel electrophoresis, an alkaline transfer, hybridisation with digoxigenin-DNA probes and detection with a chemiluminescent substrate. This method allows even low abundance mRNAs to be detected in total RNA samples from mammalian tissues.
Idioma originalInglés
Páginas (desde-hasta)263-266
PublicaciónBiotechnology Letters
Volumen23
DOI
EstadoPublicada - 28 feb 2001

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