Resumen
A rapid and sensitive non-radioactive Northern blot protocol is described which has been optimised in several critical steps. This is based on a formaldehyde-denaturing agarose gel electrophoresis, an alkaline transfer, hybridisation with digoxigenin-DNA probes and detection with a chemiluminescent substrate. This method allows even low abundance mRNAs to be detected in total RNA samples from mammalian tissues.
Idioma original | Inglés |
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Páginas (desde-hasta) | 263-266 |
Publicación | Biotechnology Letters |
Volumen | 23 |
DOI | |
Estado | Publicada - 28 feb 2001 |