TY - JOUR
T1 - Identification of Trypanosoma cruzi Discrete Typing Units (DTUs) in Latin-American migrants in Barcelona (Spain)
AU - Abras, Alba
AU - Gállego, Montserrat
AU - Muñoz, Carmen
AU - Juiz, Natalia A.
AU - Ramírez, Juan Carlos
AU - Cura, Carolina I.
AU - Tebar, Silvia
AU - Fernández-Arévalo, Anna
AU - Pinazo, María Jesús
AU - de la Torre, Leonardo
AU - Posada, Elizabeth
AU - Navarro, Ferran
AU - Espinal, Paula
AU - Ballart, Cristina
AU - Portús, Montserrat
AU - Gascón, Joaquim
AU - Schijman, Alejandro G.
PY - 2017/4/1
Y1 - 2017/4/1
N2 - Trypanosoma cruzi, the causative agent of Chagas disease, is divided into six Discrete Typing Units (DTUs): TcI-TcVI. We aimed to identify T. cruzi DTUs in Latin-American migrants in the Barcelona area (Spain) and to assess different molecular typing approaches for the characterization of T. cruzi genotypes. Seventy-five peripheral blood samples were analyzed by two real-time PCR methods (qPCR) based on satellite DNA (SatDNA) and kinetoplastid DNA (kDNA). The 20 samples testing positive in both methods, all belonging to Bolivian individuals, were submitted to DTU characterization using two PCR-based flowcharts: multiplex qPCR using TaqMan probes (MTq-PCR), and conventional PCR. These samples were also studied by sequencing the SatDNA and classified as type I (TcI/III), type II (TclI/IV) and type I/II hybrid (TcV/VI). Ten out of the 20 samples gave positive results in the flowcharts: TcV (5 samples), TcII/V/VI (3) and mixed infections by TcV plus TclI (1) and TcV plus Tcll/VI (1). By SatDNA sequencing, we classified the 20 samples, 19 as type I/II and one as type I. The most frequent DTU identified by both flowcharts, and suggested by SatDNA sequencing in the remaining samples with low parasitic loads, TcV, is common in Bolivia and predominant in peripheral blood. The mixed infection by TcV-TclI was detected for the first time simultaneously in Bolivian migrants. PCR-based flowcharts are very useful to characterize DTUs during acute infection. SatDNA sequence analysis cannot discriminate T. cruzi populations at the level of a single DTU but it enabled us to increase the number of characterized cases in chronically infected patients. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
AB - Trypanosoma cruzi, the causative agent of Chagas disease, is divided into six Discrete Typing Units (DTUs): TcI-TcVI. We aimed to identify T. cruzi DTUs in Latin-American migrants in the Barcelona area (Spain) and to assess different molecular typing approaches for the characterization of T. cruzi genotypes. Seventy-five peripheral blood samples were analyzed by two real-time PCR methods (qPCR) based on satellite DNA (SatDNA) and kinetoplastid DNA (kDNA). The 20 samples testing positive in both methods, all belonging to Bolivian individuals, were submitted to DTU characterization using two PCR-based flowcharts: multiplex qPCR using TaqMan probes (MTq-PCR), and conventional PCR. These samples were also studied by sequencing the SatDNA and classified as type I (TcI/III), type II (TclI/IV) and type I/II hybrid (TcV/VI). Ten out of the 20 samples gave positive results in the flowcharts: TcV (5 samples), TcII/V/VI (3) and mixed infections by TcV plus TclI (1) and TcV plus Tcll/VI (1). By SatDNA sequencing, we classified the 20 samples, 19 as type I/II and one as type I. The most frequent DTU identified by both flowcharts, and suggested by SatDNA sequencing in the remaining samples with low parasitic loads, TcV, is common in Bolivia and predominant in peripheral blood. The mixed infection by TcV-TclI was detected for the first time simultaneously in Bolivian migrants. PCR-based flowcharts are very useful to characterize DTUs during acute infection. SatDNA sequence analysis cannot discriminate T. cruzi populations at the level of a single DTU but it enabled us to increase the number of characterized cases in chronically infected patients. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
KW - Chagas disease
KW - Discrete Typing Unit
KW - Migration
KW - Real-time PCR
KW - Sequencing
KW - Trypanosoma cruzi
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=uab_pure&SrcAuth=WosAPI&KeyUT=WOS:000394476700012&DestLinkType=FullRecord&DestApp=WOS
U2 - 10.1016/j.parint.2016.12.003
DO - 10.1016/j.parint.2016.12.003
M3 - Article
C2 - 27940065
SN - 1383-5769
VL - 66
SP - 83
EP - 88
JO - Parasitology International
JF - Parasitology International
IS - 2
ER -