Genotoxicity of copper oxide nanoparticles in Drosophila melanogaster

Erico R. Carmona; Claudio Inostroza-Blancheteau; Veroska Obando; Laura Rubio; Ricard Marcos

doi:10.1016/j.mrgentox.2015.07.006

Genotoxicity of copper oxide nanoparticles in Drosophila melanogaster

Erico R. Carmona, Claudio Inostroza-Blancheteau, Veroska Obando, Laura Rubio, Ricard Marcos

Departamento de Genética y Microbiología

Producción científica: Contribución a una revista › Artículo › Investigación › revisión exhaustiva

47 Citas (Scopus)

Resumen

© 2015 Elsevier B.V. Copper oxide nanoparticles (CuONPs) are used as semiconductors, catalysts, gas sensors, and antimicrobial agents. We have used the comet and wing-spot assays in Drosophila melanogaster to assess the genotoxicity of CuONPs and ionic copper (CuSO<inf>4</inf>). Lipid peroxidation analysis was also performed (Thiobarbituric Acid Assay, TBARS). In larval hemocytes, both CuONPs and CuSO<inf>4</inf> caused significant dose-dependent increases in DNA damage (comet assay). In the wing-spot assay, an increase in the frequency of mutant spots was observed in the wings of the adults; CuONPs were more effective than was CuSO<inf>4</inf>. Both agents induced TBARS; again, CuONPs were more active than was CuSO<inf>4</inf>. The results indicate that CuONPs are genotoxic in Drosophila, and these effects may be mediated by oxidative stress. Most of the effects appear to be related to the presence of copper ions.

Idioma original	Inglés
Páginas (desde-hasta)	1-11
Número de páginas	11
Publicación	Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Volumen	791
DOI	https://doi.org/10.1016/j.mrgentox.2015.07.006
Estado	Publicada - sept 2015

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title = "Genotoxicity of copper oxide nanoparticles in Drosophila melanogaster",

abstract = "{\textcopyright} 2015 Elsevier B.V. Copper oxide nanoparticles (CuONPs) are used as semiconductors, catalysts, gas sensors, and antimicrobial agents. We have used the comet and wing-spot assays in Drosophila melanogaster to assess the genotoxicity of CuONPs and ionic copper (CuSO4). Lipid peroxidation analysis was also performed (Thiobarbituric Acid Assay, TBARS). In larval hemocytes, both CuONPs and CuSO4 caused significant dose-dependent increases in DNA damage (comet assay). In the wing-spot assay, an increase in the frequency of mutant spots was observed in the wings of the adults; CuONPs were more effective than was CuSO4. Both agents induced TBARS; again, CuONPs were more active than was CuSO4. The results indicate that CuONPs are genotoxic in Drosophila, and these effects may be mediated by oxidative stress. Most of the effects appear to be related to the presence of copper ions.",

keywords = "Comet assay, DNA damage, Hemocytes, Malondialdehyde, Mitotic recombination, Wing-spot test",

author = "Carmona, \{Erico R.\} and Claudio Inostroza-Blancheteau and Veroska Obando and Laura Rubio and Ricard Marcos",

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AU - Carmona, Erico R.

AU - Inostroza-Blancheteau, Claudio

AU - Obando, Veroska

AU - Rubio, Laura

AU - Marcos, Ricard

PY - 2015/9

Y1 - 2015/9

N2 - © 2015 Elsevier B.V. Copper oxide nanoparticles (CuONPs) are used as semiconductors, catalysts, gas sensors, and antimicrobial agents. We have used the comet and wing-spot assays in Drosophila melanogaster to assess the genotoxicity of CuONPs and ionic copper (CuSO4). Lipid peroxidation analysis was also performed (Thiobarbituric Acid Assay, TBARS). In larval hemocytes, both CuONPs and CuSO4 caused significant dose-dependent increases in DNA damage (comet assay). In the wing-spot assay, an increase in the frequency of mutant spots was observed in the wings of the adults; CuONPs were more effective than was CuSO4. Both agents induced TBARS; again, CuONPs were more active than was CuSO4. The results indicate that CuONPs are genotoxic in Drosophila, and these effects may be mediated by oxidative stress. Most of the effects appear to be related to the presence of copper ions.

AB - © 2015 Elsevier B.V. Copper oxide nanoparticles (CuONPs) are used as semiconductors, catalysts, gas sensors, and antimicrobial agents. We have used the comet and wing-spot assays in Drosophila melanogaster to assess the genotoxicity of CuONPs and ionic copper (CuSO4). Lipid peroxidation analysis was also performed (Thiobarbituric Acid Assay, TBARS). In larval hemocytes, both CuONPs and CuSO4 caused significant dose-dependent increases in DNA damage (comet assay). In the wing-spot assay, an increase in the frequency of mutant spots was observed in the wings of the adults; CuONPs were more effective than was CuSO4. Both agents induced TBARS; again, CuONPs were more active than was CuSO4. The results indicate that CuONPs are genotoxic in Drosophila, and these effects may be mediated by oxidative stress. Most of the effects appear to be related to the presence of copper ions.

KW - Comet assay

KW - DNA damage

KW - Hemocytes

KW - Malondialdehyde

KW - Mitotic recombination

KW - Wing-spot test

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Genotoxicity of copper oxide nanoparticles in Drosophila melanogaster

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