Expression of caprine β-lactoglobulin in the milk of transgenic mice

Elena Ibáñez*, Josep M. Folch, Francesca Vidal, Agustina Coll, Josep Santalo, Josep Egozcue, Armand Sánchez

*Autor correspondiente de este trabajo

Producción científica: Contribución a una revistaArtículoInvestigaciónrevisión exhaustiva

10 Citas (Scopus)

Resumen

A 14.5 kb-long transgene containing the complete caprine β- lactoglobulin gene transcription unit as well as 6.1 kb and 3.7 kb of the 5'- and 3'-flanking regions, respectively, was microinjected into pronuclear stage mouse embryos. Four lines of transgenic mice were obtained, three of them expressing the transgene in their mammary glands during lactation. Western blot analysis of caprine β-lactoglobulin in the milk of hemizygous transgenic animals demonstrated the presence of the exogenous protein at concentrations up to 0.5 mg ml-1 of mouse milk.
Idioma originalInglés
Páginas (desde-hasta)69-74
PublicaciónTransgenic Research
Volumen6
DOI
EstadoPublicada - 1 ene 1997

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