@article{61b6b48bfd8b4f6795325c6dbc6f86f8,
title = "Established tools and emerging trends for the production of recombinant proteins and metabolites in Pichia pastoris",
abstract = "Besides bakers' yeast, the methylotrophic yeast Komagataella phaffii (also known as Pichia pastoris) has been developed into the most popular yeast cell factory for the production of heterologous proteins. Strong promoters, stable genetic constructs and a growing collection of freely available strains, tools and protocols have boosted this development equally as thorough genetic and cell biological characterization. This review provides an overview of state-of-the-art tools and techniques for working with P. pastoris, as well as guidelines for the production of recombinant proteins with a focus on small-scale production for biochemical studies and protein characterization. The growing applications of P. pastoris for in vivo biotransformation and metabolic pathway engineering for the production of bulk and specialty chemicals are highlighted as well.",
author = "Sonakshi De and Diethard Mattanovich and Pau Ferrer and Brigitte Gasser",
note = "Funding Information: This work was supported by the Austrian BMDW and Nationalstiftung FTE through the Christian Doppler Research Association (to B.G., S.D. and D.M.)]; the BMK, BMDW, SFG, Standortagentur Tirol, Government of Lower Austria und Vienna Business Agency through the FFG – COMET Funding Program; the Austrian Science Fund FWF (BioToP) [grant number W1224]; the Spanish Ministry of Science and Innovation [grant number PID2019-104666GB-I00 (to P.F.)]; and the Agency for Management of University and Research Grants (AGAUR) of the Catalan Government - Program to support activities of research groups [grant number 2017-SGR-1462]. Publisher Copyright: {\textcopyright} 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society",
year = "2021",
month = jul,
doi = "10.1042/EBC20200138",
language = "English",
volume = "65",
pages = "293--307",
journal = "Essays in Biochemistry",
issn = "0071-1365",
publisher = "Portland Press Ltd.",
number = "2",
}