Efficient and specific transduction of cochlear supporting cells by adeno-associated virus serotype 5

Ester Ballana; Jing Wang; Frédéric Venail; Xavier Estivill; Jean Luc Puel; Maria L. Arbonès; Assumpció Bosch

doi:10.1016/j.neulet.2008.06.060

Efficient and specific transduction of cochlear supporting cells by adeno-associated virus serotype 5

Ester Ballana, Jing Wang, Frédéric Venail, Xavier Estivill, Jean Luc Puel, Maria L. Arbonès, Assumpció Bosch

Departamento de Bioquímica y Biología Molecular

Producción científica: Contribución a una revista › Artículo › Investigación › revisión exhaustiva

27 Citas (Scopus)

Resumen

Congenital deafness, affecting 1 in 1000 neonates, can lead to major problems in speech, cognitive and psychosocial development. Congenital deafness is mainly caused by mutations in connexins, hemi-channel proteins forming gap-junctions between supporting cells in the sensory epithelia. We describe a high tropism of AAV5 serotype for the supporting cells of the cochlea, both in vitro in postnatal day 4 mouse explants, and in vivo in the adult guinea-pig inner ear, through scala media perfusion. AAV5 transduction correlates with PDGFRα expression, previously reported as AAV5 receptor. This vector could be of major interest in addressing gene therapy approaches to deafness as well as for studying basic aspects of inner-ear development and hearing mechanisms. © 2008 Elsevier Ireland Ltd. All rights reserved.

Idioma original	Inglés
Páginas (desde-hasta)	134-139
Publicación	Neuroscience Letters
Volumen	442
DOI	https://doi.org/10.1016/j.neulet.2008.06.060
Estado	Publicada - 12 sept 2008

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title = "Efficient and specific transduction of cochlear supporting cells by adeno-associated virus serotype 5",

abstract = "Congenital deafness, affecting 1 in 1000 neonates, can lead to major problems in speech, cognitive and psychosocial development. Congenital deafness is mainly caused by mutations in connexins, hemi-channel proteins forming gap-junctions between supporting cells in the sensory epithelia. We describe a high tropism of AAV5 serotype for the supporting cells of the cochlea, both in vitro in postnatal day 4 mouse explants, and in vivo in the adult guinea-pig inner ear, through scala media perfusion. AAV5 transduction correlates with PDGFRα expression, previously reported as AAV5 receptor. This vector could be of major interest in addressing gene therapy approaches to deafness as well as for studying basic aspects of inner-ear development and hearing mechanisms. {\textcopyright} 2008 Elsevier Ireland Ltd. All rights reserved.",

keywords = "AAV5 serotype, Gene transfer, Inner ear, Supporting cells",

author = "Ester Ballana and Jing Wang and Fr{\'e}d{\'e}ric Venail and Xavier Estivill and Puel, {Jean Luc} and Arbon{\`e}s, {Maria L.} and Assumpci{\'o} Bosch",

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T1 - Efficient and specific transduction of cochlear supporting cells by adeno-associated virus serotype 5

AU - Ballana, Ester

AU - Wang, Jing

AU - Venail, Frédéric

AU - Estivill, Xavier

AU - Puel, Jean Luc

AU - Arbonès, Maria L.

AU - Bosch, Assumpció

PY - 2008/9/12

Y1 - 2008/9/12

N2 - Congenital deafness, affecting 1 in 1000 neonates, can lead to major problems in speech, cognitive and psychosocial development. Congenital deafness is mainly caused by mutations in connexins, hemi-channel proteins forming gap-junctions between supporting cells in the sensory epithelia. We describe a high tropism of AAV5 serotype for the supporting cells of the cochlea, both in vitro in postnatal day 4 mouse explants, and in vivo in the adult guinea-pig inner ear, through scala media perfusion. AAV5 transduction correlates with PDGFRα expression, previously reported as AAV5 receptor. This vector could be of major interest in addressing gene therapy approaches to deafness as well as for studying basic aspects of inner-ear development and hearing mechanisms. © 2008 Elsevier Ireland Ltd. All rights reserved.

AB - Congenital deafness, affecting 1 in 1000 neonates, can lead to major problems in speech, cognitive and psychosocial development. Congenital deafness is mainly caused by mutations in connexins, hemi-channel proteins forming gap-junctions between supporting cells in the sensory epithelia. We describe a high tropism of AAV5 serotype for the supporting cells of the cochlea, both in vitro in postnatal day 4 mouse explants, and in vivo in the adult guinea-pig inner ear, through scala media perfusion. AAV5 transduction correlates with PDGFRα expression, previously reported as AAV5 receptor. This vector could be of major interest in addressing gene therapy approaches to deafness as well as for studying basic aspects of inner-ear development and hearing mechanisms. © 2008 Elsevier Ireland Ltd. All rights reserved.

KW - AAV5 serotype

KW - Gene transfer

KW - Inner ear

KW - Supporting cells

U2 - 10.1016/j.neulet.2008.06.060

DO - 10.1016/j.neulet.2008.06.060

M3 - Article

SN - 0304-3940

VL - 442

SP - 134

EP - 139

JO - Neuroscience Letters

JF - Neuroscience Letters

ER -

Efficient and specific transduction of cochlear supporting cells by adeno-associated virus serotype 5

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