Distribution and genetic characterization of Enterovirus G and Sapelovirus A in six Spanish swine herds

M. J. Vilar, B. Peralta, I. García-Bocanegra, M. Simon-Grifé, A. Bensaid, J. Casal, J. Segalés, S. Pina-Pedrero

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17 Citas (Scopus)

Resumen

© 2016. The prevalence of Enterovirus G (EV-G) and Sapelovirus A (PSV-1) was investigated in Spanish swine herds by means of cross-sectional studies. Faecal samples from clinically healthy pigs were collected from six farms, and analysed by RT-PCR. The results indicated a high prevalence of EV-G detected in nearly all the animals older than 3 weeks of age. Otherwise, PSV-1 was only detected in 3-week-old piglets from one of the farms. Genetic analyses performed in the VP1 region of the EV-G indicated circulation of diverse strains in the same farm, related to genotypes G1, G2, G3, G4, G6, G9, G12, G13 and G14. Moreover, co-infection of several PSV-1 variants in the same animal was evident, typical of viral quasispecies. Evolutionary pressure analysis indicated that microevolution of PSV-1 seems to be driven by negative selection. This study gives further insights in the epidemiology of EV-G and PSV-1.
Idioma originalInglés
Páginas (desde-hasta)42-49
PublicaciónVirus Research
Volumen215
DOI
EstadoPublicada - 2 abr 2016

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