Clinical and molecular characterization of diffuse large b-cell lymphomas with 13q14.3 deletion

M. Mian, M. Scandurra, E. Chigrinova, Y. Shen, G. Inghirami, T. C. Greiner, W. C. Chan, J. M. Vose, M. Testoni, A. Chiappella, L. Baldini, M. Ponzoni, A. J.M. Ferreri, S. Franceschetti, G. Gaidano, S. Montes-Moreno, M. A. Piris, F. Facchetti, A. Tucci, J. Fr NomdedeuT. Lazure, S. Uccella, M. G. Tibiletti, E. Zucca, I. Kwee, F. Bertoni

Producción científica: Contribución a una revistaArtículoInvestigaciónrevisión exhaustiva

20 Citas (Scopus)

Resumen

Background: Deletions at 13q14.3 are common in chronic lymphocytic leukemia and are also present in diffuse large B-cell lymphomas (DLBCL) but never in immunodeficiency-related DLBCL. To characterize DLBCL with 13q14.3 deletions, we combined genome-wide DNA profiling, gene expression and clinical data in a large DLBCL series treated with rituximab, cyclophosphamide, doxorubicine, vincristine and prednisone repeated every 21 days (R-CHOP21). Patients and methods: Affymetrix GeneChip Human Mapping 250K NspI and U133 plus 2.0 gene were used. MicroRNA (miRNA) expression was studied were by real-time PCR. Median follow-up of patients was 4.9 years. Results: Deletions at 13q14.3, comprising DLEU2/MIR15A/MIR16, occurred in 22/166 (13%) cases. The deletion was wider, including also RB1, in 19/22 cases. Samples with del(13q14.3) had concomitant specific aberrations. No reduced MIR15A/MIR16 expression was observed, but 172 transcripts were significantly differential expressed. Among the deregulated genes, there were RB1 and FAS, both commonly deleted at genomic level. No differences in outcome were observed in patients treated with R-CHOP21. Conclusions: Cases with 13q14.3 deletions appear as group of DLBCL characterized by common genetic and biologic features. Deletions at 13q14.3 might contribute to DLBCL pathogenesis by two mechanisms: deregulating the cell cycle control mainly due RB1 loss and contributing to immune escape, due to FAS down-regulation. © The Author 2011. Published by Oxford University Press on behalf of the European Society for Medical Oncology. All rights reserved.
Idioma originalInglés
Páginas (desde-hasta)729-735
PublicaciónAnnals of Oncology
Volumen23
N.º3
DOI
EstadoPublicada - 1 mar 2012

Huella

Profundice en los temas de investigación de 'Clinical and molecular characterization of diffuse large b-cell lymphomas with 13q14.3 deletion'. En conjunto forman una huella única.

Citar esto