Caracterización molecular de las cepas de Klebsiella pneumoniae productoras de carbapenemasa OXA-48 tras un aumento de la resistencia a las carbapenemas en Cataluña

Marc Argente, Ana Calderón, Goretti Sauca, Montserrat Morta, Ana Vilamala, Alba Rivera, Elisenda Miró, Beatriz Mirelis, Montserrat Sierra, Carles Alonso-Tarrés, Ferran Navarro*, Carmina Martí, Carmen Gallés, Asunción Gasós, Frederic Ballester, Montserrat Olsina

*Autor correspondiente de este trabajo

Producción científica: Contribución a una revistaArtículoInvestigaciónrevisión exhaustiva

16 Citas (Scopus)
2 Descargas (Pure)

Resumen

Introduction: To characterize OXA-48 carbapenemase-producing Klebsiella pneumoniae strains isolated after an increase in carbapenem resistance in Catalonia. Methodology: K. pneumoniae identification, antimicrobial susceptibility studies, the Modified Hodge Test method, amplification of antimicrobial resistance genes (against β-lactamases, quinolones and aminoglycosides), molecular typing (by PFGE and MLST), conjugation assays, plasmid characterization (PBRT-PCR and Southern blot), a description of mobile genetic elements and statistical analysis were done. Results: OXA-48 was the only carbapenemase detected, with a prevalence of 1.9%. The blaOXA-48 gene was located in an IncL conjugative plasmid of 62 kb and integrated into the transposons Tn1999.2 (91.7%) or Tn1999.1. Five PFGE profiles (A to E) were found, which exactly matched the MLST: ST101, ST17, ST1233, ST14 and ST405, respectively. ST1233 is described here for the first time. K. pneumoniae OXA-48-producing strains were also CTX-M-15 carriers, some producing OXA-1 and TEM-1 penicillinases. The acquired qnrB66 and qnrB1 and aac(3′)-IIa, aac(6′)-Ib genes were also identified. Conclusion: The K. pneumoniae ST405 clone has played an important role in the growing prevalence of OXA-48 in Catalonia. All clones described preserved the blaOXA-48 genetic environment and mobile genetic elements (Tn1999). Notably, the three strains with minor sequence types in this study are not multiresistant strains. These strains are expanding in elderly patients (average age of 76 years) with serious underlying diseases, mainly women (61.2%).

Título traducido de la contribuciónMolecular characterization of OXA-48 carbapenemase-producing Klebsiella pneumoniae strains after a carbapenem resistance increase in Catalonia
Idioma originalEspañol
Páginas (desde-hasta)82-88
Número de páginas7
PublicaciónEnfermedades Infecciosas y Microbiologia Clinica
Volumen37
N.º2
DOI
EstadoPublicada - feb 2019

Palabras clave

  • PFGE
  • MLST
  • Antibiotic resistance
  • Carbapenemase
  • OXA-48

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