TY - CHAP
T1 - Bacterial inclusion body purification
AU - Seras-Franzoso, Joaquin
AU - Peternel, Spela
AU - Cano-Garrido, Olivia
AU - Villaverde, Antonio
AU - García-Fruitós, Elena
PY - 2014/12/1
Y1 - 2014/12/1
N2 - © Springer Science+Business Media New York 2015. All rights are reserved. Purification of bacterial inclusion bodies (IBs) is gaining importance due to the raising of novel applications for this type of submicron particulate protein clusters, with potential uses in the biomedical field among others. Here, we present two optimized methods to purify IBs adapting classical procedures to the material nature as well as the requirements of its final application.
AB - © Springer Science+Business Media New York 2015. All rights are reserved. Purification of bacterial inclusion bodies (IBs) is gaining importance due to the raising of novel applications for this type of submicron particulate protein clusters, with potential uses in the biomedical field among others. Here, we present two optimized methods to purify IBs adapting classical procedures to the material nature as well as the requirements of its final application.
KW - Bacterial cell free
KW - Bacterial inclusion body
KW - Cell disruption
KW - Nanoparticles
KW - Purification
U2 - 10.1007/978-1-4939-2205-5_16
DO - 10.1007/978-1-4939-2205-5_16
M3 - Chapter
SN - 9781493922055
SN - 9781493922048
SP - 293
EP - 305
BT - Insoluble Proteins: Methods and Protocols
ER -