TY - JOUR
T1 - Aberrant expression of epithelial leucine-rich repeat containing G protein–coupled receptor 5–positive cells in the eutopic endometrium in endometriosis and implications in deep-infiltrating endometriosis
AU - Vallvé-Juanico, Júlia
AU - Suárez-Salvador, Elena
AU - Castellví, Josep
AU - Ballesteros, Agustín
AU - Taylor, Hugh S.
AU - Gil-Moreno, Antonio
AU - Santamaria, Xavier
PY - 2017/11/1
Y1 - 2017/11/1
N2 - © 2017 Objective To characterize leucine-rich repeat containing G protein-coupled receptor 5–positive (LGR5+) cells from the endometrium of women with endometriosis. Design Prospective experimental study. Setting University hospital/fertility clinic. Patient(s) Twenty-seven women with endometriosis who underwent surgery and 12 healthy egg donors, together comprising 39 endometrial samples. Intervention(s) Obtaining of uterine aspirates by using a Cornier Pipelle. Main Outcomes Measure(s) Immunofluorescence in formalin-fixed paraffin-embedded tissue from mice and healthy and pathologic human endometrium using antibodies against LGR5, E-cadherin, and cytokeratin, and epithelial and stromal LGR5+ cells isolated from healthy and pathologic human eutopic endometrium by fluorescence-activated cell sorting and transcriptomic characterization by RNA high sequencing. Result(s) Immunofluorescence showed that LGR5+ cells colocalized with epithelial markers in the stroma of the endometrium only in endometriotic patients. The results from RNA high sequencing of LGR5+ cells from epithelium and stroma did not show any statistically significant differences between them. The LGR5+ versus LGR5− cells in pathologic endometrium showed 394 differentially expressed genes. The LGR5+ cells in deep-infiltrating endometriosis expressed inflammatory markers not present in the other types of the disease. Conclusion(s) Our results revealed the presence of aberrantly located LGR5+ cells coexpressing epithelial markers in the stromal compartment of women with endometriosis. These cells have a statistically significantly different expression profile in deep-infiltrating endometriosis in comparison with other types of endometriosis, independent of the menstrual cycle phase. Further studies are needed to elucidate their role and influence in reproductive outcomes.
AB - © 2017 Objective To characterize leucine-rich repeat containing G protein-coupled receptor 5–positive (LGR5+) cells from the endometrium of women with endometriosis. Design Prospective experimental study. Setting University hospital/fertility clinic. Patient(s) Twenty-seven women with endometriosis who underwent surgery and 12 healthy egg donors, together comprising 39 endometrial samples. Intervention(s) Obtaining of uterine aspirates by using a Cornier Pipelle. Main Outcomes Measure(s) Immunofluorescence in formalin-fixed paraffin-embedded tissue from mice and healthy and pathologic human endometrium using antibodies against LGR5, E-cadherin, and cytokeratin, and epithelial and stromal LGR5+ cells isolated from healthy and pathologic human eutopic endometrium by fluorescence-activated cell sorting and transcriptomic characterization by RNA high sequencing. Result(s) Immunofluorescence showed that LGR5+ cells colocalized with epithelial markers in the stroma of the endometrium only in endometriotic patients. The results from RNA high sequencing of LGR5+ cells from epithelium and stroma did not show any statistically significant differences between them. The LGR5+ versus LGR5− cells in pathologic endometrium showed 394 differentially expressed genes. The LGR5+ cells in deep-infiltrating endometriosis expressed inflammatory markers not present in the other types of the disease. Conclusion(s) Our results revealed the presence of aberrantly located LGR5+ cells coexpressing epithelial markers in the stromal compartment of women with endometriosis. These cells have a statistically significantly different expression profile in deep-infiltrating endometriosis in comparison with other types of endometriosis, independent of the menstrual cycle phase. Further studies are needed to elucidate their role and influence in reproductive outcomes.
KW - Deep infiltrating endometriosis (DIE)
KW - LGR5
KW - epithelial mesenchymal transition (EMT)
KW - macrophages
KW - uterine aspirate
U2 - 10.1016/j.fertnstert.2017.08.018
DO - 10.1016/j.fertnstert.2017.08.018
M3 - Article
SN - 0015-0282
VL - 108
SP - 858-867.e2
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 5
ER -
