TY - JOUR
T1 - A novel activity of immobilized penicillin G acylase: Removal of benzyloxycarbonyl amino protecting group
AU - ALVARO, G
AU - FELIU, JA
AU - CAMINAL, G
AU - LOPEZ-SANTIN, J
AU - CLAPES, P
PY - 2000
Y1 - 2000
N2 - A novel activity of penicillin G acylase from E. coli is presented. This enzyme, immobilized onto agarose gels by multipoint covalent attachment, was used for removing the benzyloxycarbonyl (Z) amino protecting group from selected amino acids and oligopeptides in different reaction systems. Quantitative deprotection yields were obtained for simple amino acids, while the efficiency with oligopeptides varied depending on the sequence.
AB - A novel activity of penicillin G acylase from E. coli is presented. This enzyme, immobilized onto agarose gels by multipoint covalent attachment, was used for removing the benzyloxycarbonyl (Z) amino protecting group from selected amino acids and oligopeptides in different reaction systems. Quantitative deprotection yields were obtained for simple amino acids, while the efficiency with oligopeptides varied depending on the sequence.
U2 - 10.3109/10242420009015248
DO - 10.3109/10242420009015248
M3 - Article
SN - 0886-4454
SP - 253
EP - 258
JO - Biocatalysis and Biotransformation
JF - Biocatalysis and Biotransformation
ER -