TY - JOUR
T1 - A new rapid and more sensitive microcytotoxicity test
AU - García, Pedro
AU - Rodriguez, JoséLuis
AU - Viñas, José
PY - 1972/1/1
Y1 - 1972/1/1
N2 - A new rapid microcytotoxicity test based on the use of ethidium bromide is described. This substance diffuses rapidly through the membrane of damaged cells and gives an intense red fluorescence with the nuclear DNA. The results can be read in a regular fluorescence microscope 30 min after addition of the cell suspension to standard microtrays, and are easier to interpret than the currently used microcytotoxicity tests. With longer incubation times this method permits the use of dilute HLA typing sera. It is speculated that this would open the way to the preparation of absorbed HLA sera as reagents. © 1972.
AB - A new rapid microcytotoxicity test based on the use of ethidium bromide is described. This substance diffuses rapidly through the membrane of damaged cells and gives an intense red fluorescence with the nuclear DNA. The results can be read in a regular fluorescence microscope 30 min after addition of the cell suspension to standard microtrays, and are easier to interpret than the currently used microcytotoxicity tests. With longer incubation times this method permits the use of dilute HLA typing sera. It is speculated that this would open the way to the preparation of absorbed HLA sera as reagents. © 1972.
UR - https://www.scopus.com/pages/publications/0015342194
U2 - 10.1016/0022-1759(72)90007-5
DO - 10.1016/0022-1759(72)90007-5
M3 - Article
SN - 0022-1759
VL - 1
SP - 303
EP - 307
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 3
ER -