A hyaluronic acid demilune scaffold and polypyrrole-coated fibers carrying embedded human neural precursor cells and curcumin for surface capping of spinal cord injuries

Hoda Elkhenany, Pablo Bonilla, Esther Giraldo, Ana Alastrue-Agudo, Michael J Edel, María Jesús Vicent, Fernando Gisbert Roca, Cristina Marínez Ramos, Laura Rodríguez Doblado, Manuel Monleón Pradas, Victoria Moreno Manzano

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17 Citas (Scopus)

Resumen

Tissue engineering, including cell transplantation and the application of biomaterials and bioactive molecules, represents a promising approach for regeneration following spinal cord injury (SCI). We designed a combinatorial tissue-engineered approach for the minimally invasive treatment of SCI-a hyaluronic acid (HA)-based scaffold containing polypyrrole-coated fibers (PPY) combined with the RAD16-I self-assembling peptide hydrogel (Corning® PuraMatrix™ peptide hydrogel (PM)), human induced neural progenitor cells (iNPCs), and a nanoconjugated form of curcumin (CURC). In vitro cultures demonstrated that PM preserves iNPC viability and the addition of CURC reduces apoptosis and enhances the outgrowth of Nestin-positive neurites from iNPCs, compared to non-embedded iNPCs. The treatment of spinal cord organotypic cultures also demonstrated that CURC enhances cell migration and prompts a neuron-like morphology of embedded iNPCs implanted over the tissue slices. Following sub-acute SCI by traumatic contusion in rats, the implantation of PM-embedded iNPCs and CURC with PPY fibers supported a significant increase in neuro-preservation (as measured by greater βIII-tubulin staining of neuronal fibers) and decrease in the injured area (as measured by the lack of GFAP staining). This combination therapy also restricted platelet-derived growth factor expression, indicating a reduction in fibrotic pericyte invasion. Overall, these findings support PM-embedded iNPCs with CURC placed within an HA demilune scaffold containing PPY fibers as a minimally invasive combination-based alternative to cell transplantation alone.
Idioma originalInglés
PublicaciónBiomedicines
Volumen9
N.º12
DOI
EstadoPublicada - 2021

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