Additional file 7: Figure S7. of Quaternary structure of a G-protein-coupled receptor heterotetramer in complex with Gi and Gs

Molecular dynamics (MD) simulation of the adenosine A1R-A2AR heterotetramer in complex with Gi and Gs. (A) Root-mean-square deviations (rmsd) on protein α-carbons of the whole system (black solid line), of the two A1Rs (orange and red solid lines), of the two A2ARs (light and dark green solid lines), of Gi (gray solid line), and of Gs (gray dotted line) throughout the MD simulation. This color scheme matches with the color of the different proteins depicted in the two adjacent schematic representations. (B) Intermolecular distances between the N-terminal helices of the γ-subunit of Gi and Gs (magenta line), the N-terminal helices of the α-subunit of Gi and Gs (gray line), the N-terminal helix of the α-subunit of Gi and the C-terminal helix (Hx8) of inactive A1R (orange line), the N-terminal helix of the α-subunit of Gs and the C-terminal Hx8 of inactive A2AR (green line), the C-terminal Hx8 of A1R and A2AR (blue lines). These computed intermolecular distances are depicted as double arrows in the two adjacent schematic representations. (TIF 6973 kb)