NANOSISTEMAS AVANZADOS PARA APLICACIONES DERMATOLÓGICAS

Abstract

The emergence of nanotechnology in life sciences has generated high expectations due to its promising diagnostic, therapeutic and cosmetic applications. One of the major challenges of this process lies in the development of "nanotherapies", that are specifically targeted to destined tissues and organs avoiding the systemic adverse effects of current treatments._x000D_ Because of their biocompatibility, gold nanoparticles (AuNPs) are among the most widely used metal nanoparticles on the research of new biomedical products. AuNPs possess a reactive surface that allows the attachment of organic molecules and exhibit interesting optical properties, such as the surface plasmon resonance, which facilitate the design of advanced systems with multiple applications. Several studies have shown that AuNPs conjugated to stabilizing molecules (such as polyethylene glycol) express very low levels of toxicity, lower than precursor gold salts. On the other hand, hyaluronic acid (HA) is broadly used in medicine, especially in dermatology. HA has structural functions and it is involved in the maintenance of homeostasis, playing an important role in certain tissues such as skin, cartilage and joints. In addition, HA acts on cellular level through binding to different receptors such as CD44, Rhamm, LYVE1 and HARE. Through its main receptor CD44, HA has been shown to be involved in migration, cell proliferation, survival and differentiation processes. HA is an essential ingredient of cosmetic products and its use has been proven to promote skin regeneration despite being a large molecule that can not penetrate the deep layers of the skin._x000D_ During this project, we have developed an advanced nanosystem called Golden Hyaluronan (GH), consisting of a spherical core of gold nanoparticle conjugated to modified small size HA molecules in a stable manner; thus, facilitating deep skin layer penetration. We have also studied both structural and cellular effects produced by GH, showing that it successfully passes in vitro toxicity, genotoxicity, and efficacy tests as well as in vivo toxicity and efficacy trials._x000D_ Our studies with GH have shown a safety health profiling as well as better effects on efficacy compared to HA. We have shown that GH has stimulated the synthesis of different molecules involved in regeneration processes especially the synthesis of molecules with cosmetic interest, such as HA, collagen, and elastin, confirming our hypothesis. Thereby, utilizing the results of this thesis, Endor has developed a pioneering product with dermocosmetic applications in the field of skin regeneration and rejuvenation, called Y.en Effect.

Date of Award	20 Oct 2017
Original language	Spanish
Supervisor	Judith Sendra Cuadal (Director)