Zymobacter palmae pyruvate decarboxylase production process development: Cloning in Escherichia coli, fed-batch culture and purification

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Abstract

© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Pyruvate decarboxylase (PDC) is responsible for the decarboxylation of pyruvate, producing acetaldehyde and carbon dioxide and is of high interest for industrial applications. PDC is a very powerful tool in the enzymatic synthesis of chiral amines by combining it with transaminases when alanine is used as amine donor. However, one of the main drawback that hampers its use in biocatalysis is its production and the downstream processing on scale. In this paper, a production process of PDC from Zymobacter palmae has been developed. The enzyme has been cloned and overexpressed in Escherichia coli. It is presented, for the first time, the evaluation of the production of recombinant PDC in a bench-scale bioreactor, applying a substrate-limiting fed-batch strategy which led to a volumetric productivity and a final PDC specific activity of 6942 U L−1h−1 and 3677 U gDCW−1 (dry cell weight). Finally, PDC was purified in fast protein liquid chromatography equipment by ion exchange chromatography. The developed purification process resulted in 100% purification yield and a purification factor of 3.8.
Original languageEnglish
Pages (from-to)502-512
Number of pages11
JournalEngineering in Life Sciences
Volume19
Issue number7
DOIs
Publication statusPublished - 1 Jul 2019

Keywords

  • PDC purification
  • Zymobacter palmae
  • fed-batch culture
  • high cell density cultures
  • pyruvate decarboxylase (PDC)

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