ZmMYB31 directly represses maize lignin genes and redirects the phenylpropanoid metabolic flux

Silvia Fornalé, Xinhui Shi, Chenglin Chai, Antonio Encina, Sami Irar, Montserrat Capellades, Elisabet Fuguet, Josep Lluís Torres, Pere Rovira, Pere Puigdomènech, Joan Rigau, Erich Grotewold, John Gray, David Caparrós-Ruiz

    Research output: Contribution to journalArticleResearchpeer-review

    172 Citations (Scopus)

    Abstract

    Few regulators of phenylpropanoids have been identified in monocots having potential as biofuel crops. Here we demonstrate the role of the maize (Zea mays) R2R3-MYB factor ZmMYB31 in the control of the phenylpropanoid pathway. We determined its in vitro consensus DNA-binding sequence as ACC T/ AACC, and chromatin immunoprecipitation (ChIP) established that it interacts with two lignin gene promoters in vivo. To explore the potential of ZmMYB31 as a regulator of phenylpropanoids in other plants, its role in the regulation of the phenylpropanoid pathway was further investigated in Arabidopsis thaliana. ZmMYB31 downregulates several genes involved in the synthesis of monolignols and transgenic plants are dwarf and show a significantly reduced lignin content with unaltered polymer composition. We demonstrate that these changes increase cell wall degradability of the transgenic plants. In addition, ZmMYB31 represses the synthesis of sinapoylmalate, resulting in plants that are more sensitive to UV irradiation, and induces several stress-related proteins. Our results suggest that, as an indirect effect of repression of lignin biosynthesis, transgenic plants redirect carbon flux towards the biosynthesis of anthocyanins. Thus, ZmMYB31 can be considered a good candidate for the manipulation of lignin biosynthesis in biotechnological applications. © 2010 Blackwell Publishing Ltd.
    Original languageEnglish
    Pages (from-to)633-644
    JournalPlant Journal
    Volume64
    Issue number4
    DOIs
    Publication statusPublished - 1 Nov 2010

    Keywords

    • cell wall
    • flavonoids
    • lignin
    • maize
    • phenylpropanoids
    • R2R3-MYB

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