White-rot fungus-mediated degradation of the analgesic ketoprofen and identification of intermediates by HPLC-DAD-MS and NMR

Ernest Marco-Urrea, Miriam Pérez-Trujillo, Carles Cruz-Morató, Gloria Caminal, Teresa Vicent

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    Abstract

    Ketoprofen is a nonsteroidal anti-inflammatory drug that has been detected in the environment in the range of ng L-1-μg L-1 due to its low degradability in some wastewater treatment plants. In this study, the use of the white-rot fungus Trametes versicolor to effectively degrade ketoprofen in a defined liquid medium was assessed. The fungus eliminated ketoprofen to nondetectable levels in 24 h when it was added at 10 mg L-1 whereas at low concentration of 40 μg L-1 it was almost completely removed (95%) after 5 h. Low extracellular laccase activity was detected in the T. versicolor cultures but the addition of the laccase-mediator system did not lead to ketoprofen oxidation. The cytochrome P-450 inhibitor 1-aminobenzotriazole reduced ketoprofen oxidation. These data suggest that the first oxidation step is cytochrome P450 mediated. During time-course degradation experiments, three intermediates were structurally elucidated and quantified by HPLC-DAD-MS and NMR: 2-[3-(4-hydroxybenzoyl)phenyl]-propanoic acid, 2-[(3-hydroxy(phenyl)methyl)phenyl]-propanoic acid, and 2-(3-benzoyl-4-hydroxyphenyl)-propanoic acid. The latter was reported for the first time in biological systems. After 7 d of incubation, only small amounts of 2-[(3-hydroxy(phenyl)methyl)phenyl]-propanoic acid (0.08 mg) remained in the liquid medium in comparison with the initial ketoprofen dose (1.0 mg), suggesting possible mineralization of ketoprofen. © 2009 Elsevier Ltd. All rights reserved.
    Original languageEnglish
    Pages (from-to)474-481
    JournalChemosphere
    Volume78
    DOIs
    Publication statusPublished - 1 Jan 2010

    Keywords

    • Biodegradation
    • HPLC-DAD-MS
    • Ketoprofen
    • NMR
    • Trametes versicolor

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