© 2018 Elsevier Inc. Living kidney related donations (LKRD) should be promoted because of the current deficit of organs for transplantation. The objective of the study was to analyze the attitude of Spanish veterinary students toward LKRD, because they may influence public opinion in the future, and to determine the factors that condition it. Methods: A sociological, interdisciplinary, multicenter, and observational study was carried out in the veterinary students enrolled in Spain (n = 9000) in the university academic year 2010–2011. A sample of 2815 students was stratified by geographical area and academic year. The students’ attitude toward LKRD was assessed using a psychosocial validated questionnaire (PCID-DVR Rios), was self-administered, and was completed anonymously. Veterinary schools were randomly selected. The questionnaire was applied to each academic year at compulsory sessions. Statistical analysis was preformed using t test, χ2 test, and logistic regression analysis. Results: The completion rate was 95% (n = 2683); 93% (n = 2504) were in favor of LKRD and 36% (n = 945) supported unrelated living kidney donation. The following variables were associated with having a more favorable attitude: (1) sex (P <.001), (2) being a student of southern universities (P =.03), (3) attitude toward deceased donation (P <.001), (4) having a father (P <.001) or a mother (P <.001) in favor of organ donation, and (5) having discussed the subject with friends (P =.03) or family (P =.02). However, only 60% would accept a kidney from a relative. Conclusion: Spanish veterinary students have a favorable attitude toward LKRD. However, only 60% would accept a kidney from a relative.