Variations in cell size and buoyant density of Escherichia coli K12 during glycogen accumulation

Jordi Mas; Carlos Pedrós-Alió; Ricardo Guerrero

doi:10.1111/j.1574-6968.1989.tb03305.x

Variations in cell size and buoyant density of Escherichia coli K12 during glycogen accumulation

Jordi Mas, Carlos Pedrós-Alió, Ricardo Guerrero

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Abstract

The effect of glycogen accumulation on buoyant density and volume of Escherichia coli K12 was studied. A procedure consisting of three linear equations is presented. This requires measurement only of three parameters: cell buoyant density, cell volume and specific content of the polymer. Experimental values are then used to calculate intercepts and slopes of the equations by linear regression. From the estimated values of such parameters the in vivo values of several variables of interest can be calculated. These include in vivo density and volume of the glycogen inclusion, as well as density and volume of the structural material in the cell. The results are consistent with the glycogen inclusions being hydrated. © 1989.

Original language	English
Pages (from-to)	231-236
Journal	FEMS Microbiology Letters
Volume	57
Issue number	2
DOIs	https://doi.org/10.1111/j.1574-6968.1989.tb03305.x
Publication status	Published - 15 Jan 1989

Keywords

Cell buoyant density
Cell size
Escherichia coli
Glycogen
Macromolecular composition

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10.1111/j.1574-6968.1989.tb03305.x

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title = "Variations in cell size and buoyant density of Escherichia coli K12 during glycogen accumulation",

abstract = "The effect of glycogen accumulation on buoyant density and volume of Escherichia coli K12 was studied. A procedure consisting of three linear equations is presented. This requires measurement only of three parameters: cell buoyant density, cell volume and specific content of the polymer. Experimental values are then used to calculate intercepts and slopes of the equations by linear regression. From the estimated values of such parameters the in vivo values of several variables of interest can be calculated. These include in vivo density and volume of the glycogen inclusion, as well as density and volume of the structural material in the cell. The results are consistent with the glycogen inclusions being hydrated. {\textcopyright} 1989.",

keywords = "Cell buoyant density, Cell size, Escherichia coli, Glycogen, Macromolecular composition",

author = "Jordi Mas and Carlos Pedr{\'o}s-Ali{\'o} and Ricardo Guerrero",

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T1 - Variations in cell size and buoyant density of Escherichia coli K12 during glycogen accumulation

AU - Mas, Jordi

AU - Pedrós-Alió, Carlos

AU - Guerrero, Ricardo

PY - 1989/1/15

Y1 - 1989/1/15

N2 - The effect of glycogen accumulation on buoyant density and volume of Escherichia coli K12 was studied. A procedure consisting of three linear equations is presented. This requires measurement only of three parameters: cell buoyant density, cell volume and specific content of the polymer. Experimental values are then used to calculate intercepts and slopes of the equations by linear regression. From the estimated values of such parameters the in vivo values of several variables of interest can be calculated. These include in vivo density and volume of the glycogen inclusion, as well as density and volume of the structural material in the cell. The results are consistent with the glycogen inclusions being hydrated. © 1989.

AB - The effect of glycogen accumulation on buoyant density and volume of Escherichia coli K12 was studied. A procedure consisting of three linear equations is presented. This requires measurement only of three parameters: cell buoyant density, cell volume and specific content of the polymer. Experimental values are then used to calculate intercepts and slopes of the equations by linear regression. From the estimated values of such parameters the in vivo values of several variables of interest can be calculated. These include in vivo density and volume of the glycogen inclusion, as well as density and volume of the structural material in the cell. The results are consistent with the glycogen inclusions being hydrated. © 1989.

KW - Cell buoyant density

KW - Cell size

KW - Escherichia coli

KW - Glycogen

KW - Macromolecular composition

U2 - 10.1111/j.1574-6968.1989.tb03305.x

DO - 10.1111/j.1574-6968.1989.tb03305.x

M3 - Article

VL - 57

SP - 231

EP - 236

IS - 2

ER -

Variations in cell size and buoyant density of Escherichia coli K12 during glycogen accumulation

Abstract

Keywords

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