Variations in cell size and buoyant density of Escherichia coli K12 during glycogen accumulation

Jordi Mas, Carlos Pedrós-Alió, Ricardo Guerrero

    Research output: Contribution to journalArticleResearchpeer-review

    3 Citations (Scopus)

    Abstract

    The effect of glycogen accumulation on buoyant density and volume of Escherichia coli K12 was studied. A procedure consisting of three linear equations is presented. This requires measurement only of three parameters: cell buoyant density, cell volume and specific content of the polymer. Experimental values are then used to calculate intercepts and slopes of the equations by linear regression. From the estimated values of such parameters the in vivo values of several variables of interest can be calculated. These include in vivo density and volume of the glycogen inclusion, as well as density and volume of the structural material in the cell. The results are consistent with the glycogen inclusions being hydrated. © 1989.
    Original languageEnglish
    Pages (from-to)231-236
    JournalFEMS Microbiology Letters
    Volume57
    Issue number2
    DOIs
    Publication statusPublished - 15 Jan 1989

    Keywords

    • Cell buoyant density
    • Cell size
    • Escherichia coli
    • Glycogen
    • Macromolecular composition

    Fingerprint Dive into the research topics of 'Variations in cell size and buoyant density of Escherichia coli K12 during glycogen accumulation'. Together they form a unique fingerprint.

    Cite this