The structure of the glacial ocean was significantly different to that of the present day with intermediate to mid-depth waters being notably more δ13C enriched than deep waters. This contrast was especially pronounced in the South Atlantic suggesting the development of a sharp chemical divide, or 'chemocline', at around 2500 m water depth between upper and lower layers, with implications for deep-ocean carbon storage [Hodell et al., 2003. Pleistocene vertical carbon isotope and carbonate gradients in the South Atlantic sector of the Southern Ocean. Geochemistry, Geophysics, Geosystems, 4(1): doi: 1004 10.1029/2002GC000367.]. We evaluate existing benthic foraminiferal δ13C, Cd/Ca and derived carbon isotope air-sea exchange signature (δ13Cas) data sets for the Atlantic during the Last Glacial Maximum (LGM), and Marine Isotope Stages (MIS) 6 and 8 in order to examine the regional extent of the chemocline in the South Atlantic. Benthic δ13C data north of the approximate latitude of the LGM Subantarctic Front (LGM-SAF, 43°S) linearly decrease with water depth, indicative of mixing between upper 'well' and lower 'poorly' ventilated water masses, with little evidence of the sharp chemical divide. Conversely, benthic δ13C data south of the LGM-SAF below 2500 m water depth are uniformly around -0.8‰. The apparent δ13C gradient across the LGM-SAF suggests enhanced mid-depth ventilation north of the SAF and reduced ventilation to the south. From this pattern we conclude that the regional chemocline in the South Atlantic constituted a dominantly meridional, rather than a vertical gradient, and was developed during at least the past three glacial periods. Benthic Cd/Ca data indicate that the gradient was not nutrient related, although further data from the South Atlantic are needed for a better assessment of this suggestion. The combined benthic δ13C and Cd/Ca data indicate the source of well-ventilated upper waters in the South Atlantic changed from Northern Component Water (NCW) during early glacial phases to Upper Southern Component Water (USCW) during mid-to-late glacial phases when the Southern Ocean may have become isolated. USCW maintained a positive δ13C and δ13Cas signature simulating a North Atlantic origin that has been implicated in previous studies. The data demonstrate that secular imprints on δ13C must be taken into consideration when assessing the implications of the vertical δ13C gradient. This data also supports a variable water column architecture and modes of water mass formation as primary means to draw down atmospheric CO2 and storage in the abyssal ocean by involving processes occurring on either side of the SAF in the glacial Southern Ocean. Crown Copyright © 2009.