Vanadate raises fructose 2,6-bisphosphate concentrations and activates glycolysis in rat hepatocytes

A. M. Gomez-Foix, J. E. Rodriguez-Gil, C. Fillat, J. J. Guinovart, F. Bosch

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35 Citations (Scopus)

Abstract

In rat hepatocytes, vanadate increases fructose 2,6-bisphosphate (Fru-2,6-P2) in a time- and dose-dependent manner, and counteracts the decrease in this metabolite caused by glucagon, forskolin or exogenous cyclic AMP. Vanadate does not directly modify the activity of 6-phosphofructo-2-kinase, even though it can counteract the inactivation of this enzyme caused by glucagon. Furthermore, vanadate raises the yield of 3H2O from [3-3H]glucose, indicating that it increases the flux through 6-phosphofructo-1-kinase. Moreover, vanadate in hepatocytes incubated in the presence of glucose increases the production of both lactate and CO2. Therefore vanadate has insulin-like effects on the glycolytic pathway in rat hepatocytes. These results clearly contrast with our previous observation that vanadate exerts glycogenolytic non-insulin-like effects on glycogen synthase and phosphorylase.
Original languageEnglish
Pages (from-to)507-512
JournalBiochemical Journal
Volume255
Issue number2
Publication statusPublished - 1 Jan 1988

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