Value of p16INK4aand Ki-67 immunohistochemical staining in cervical intraepithelial neoplasia grade 2 biopsies as biomarkers for cervical intraepithelial neoplasia grade 3 in cone results

Ester Miralpeix, Josep M. Solé-Sedeño, Gemma Mancebo, Belen Lloveras, Beatriz Bellosillo, Ramon Carreras, Francesc Alameda

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Abstract

© Science Printers and Publishers, Inc. OBJECTIVE: To evaluate the usefulness of p16INK4a(p16) and Ki-67 staining in high-grade cervical intraepithelial neoplasia (CIN2) biopsies in order to predict CIN3 results in cone specimens, thereby sparing those not likely at risk for CIN3 from unnecessary cone excision. STUDY DESIGN: We retrospectively recruited patients with CIN2 colposcopy-directed biopsy treated by loop electrosurgical excision procedure. The expression of p16 and Ki-67 was qualitatively and quantitatively analyzed in all biopsies and cone specimens. RESULTS: A total of 123 patients from January 2009 to December 2010 were included in the study. CIN3 incone specimens was observed in 35 patients (28.5%). Ki-67 positive immunostaining in >50% of epithelial cells was related to CIN3 diagnoses in cone specimens (p=0.043). However, p16+ and Ki-67+ evaluated by thirds of the epithelial thickness in CIN2 biopsies did not show a significant correlation with the cone results. In multivariate analysis, Ki-67 cell expression over 50% in CIN2 biopsies and high-grade squamous intraepithelial lesion (HSIL) in the previous cytology were statistically associated with CIN3 results in the cone (odds ratio [OR] 2.55, 95% confidence interval [CI] 1.04-6.29; OR 2.68, 95% CI 1.07-6.72, respectively). CONCLUSION: Patients with HSIL in the previous cytology and Ki-67 cell expression over 50% in their CIN2 biopsies could be considered in need of treatment by cone for their higher risk of underlying CIN3 lesions.
Original languageEnglish
Pages (from-to)1-8
JournalAnalytical and Quantitative Cytology and Histology
Volume38
Issue number1
Publication statusPublished - 1 Feb 2016

Keywords

  • Cervical intraepithelial neoplasia 2
  • Cervical intraepithelial neoplasia 3
  • CIN 2
  • CIN 3
  • Highgrade squamous intraepithelial lesion
  • HSIL
  • Immunohistochemistry
  • Ki-67
  • p16
  • p16 INK4a

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    Miralpeix, E., Solé-Sedeño, J. M., Mancebo, G., Lloveras, B., Bellosillo, B., Carreras, R., & Alameda, F. (2016). Value of p16INK4aand Ki-67 immunohistochemical staining in cervical intraepithelial neoplasia grade 2 biopsies as biomarkers for cervical intraepithelial neoplasia grade 3 in cone results. Analytical and Quantitative Cytology and Histology, 38(1), 1-8.