TY - JOUR
T1 - Valorization of agro-industrial wastes by producing 2-phenylethanol via solid-state fermentation
T2 - Influence of substrate selection on the process
AU - Martínez-Avila, Oscar
AU - Muñoz-Torrero, Patricia
AU - Sánchez, Antoni
AU - Font, Xavier
AU - Barrena, Raquel
N1 - Funding Information:
This work was supported by the Spanish Ministerio de Economía y Competitividad (Project CTM2015-69513-R).
Publisher Copyright:
© 2021 Elsevier Ltd
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/2/15
Y1 - 2021/2/15
N2 - 2-phenylethanol (2-PE) is a value-added compound widely used in industry due to its rose-like odor and antibacterial properties that can be bioproduced using wastes as raw materials. This study presents the valorization of nine agro-industrial wastes as potential substrates for 2-PE production using an isolated 2-PE producer Pichia kudriavzevii, and the solid-state fermentation (SSF) technology as an alternative approach. The assessed substrates comprised wastes of varied traits such that each of them provided different characteristics to the fermentation. Thus, by using a principal component analysis (PCA), it was possible to identify the most significant characteristics associated with the substrates affecting the 2-PE production. Results show that L-phenylalanine biotransformation was more efficient than de novo synthesis for producing 2-PE. Besides, from the evaluated set, the maximum 2-PE production was achieved with red apple pomace, reaching 1.7 and 25.2 mg2PE per gram of used waste through de novo and L-phenylalanine biotransformation, respectively. In that scenario, volumetric productivity and precursor yield were 39.6 mg2PE L-1h−1 and 0.69 g2PE per gram of L-phenylalanine added, respectively. From the PCA, it was identified that the reducing sugars content of the substrate, the air-filled porosity of the bed and the L-phenylalanine availability were the most critical parameters (associated with the substrates) influencing the microbial activity and 2-PE production. These results suggest that the desirable traits a solid media needs for promoting 2-PE production via SSF could be reached by using a combination of wastes in a synergistic approach.
AB - 2-phenylethanol (2-PE) is a value-added compound widely used in industry due to its rose-like odor and antibacterial properties that can be bioproduced using wastes as raw materials. This study presents the valorization of nine agro-industrial wastes as potential substrates for 2-PE production using an isolated 2-PE producer Pichia kudriavzevii, and the solid-state fermentation (SSF) technology as an alternative approach. The assessed substrates comprised wastes of varied traits such that each of them provided different characteristics to the fermentation. Thus, by using a principal component analysis (PCA), it was possible to identify the most significant characteristics associated with the substrates affecting the 2-PE production. Results show that L-phenylalanine biotransformation was more efficient than de novo synthesis for producing 2-PE. Besides, from the evaluated set, the maximum 2-PE production was achieved with red apple pomace, reaching 1.7 and 25.2 mg2PE per gram of used waste through de novo and L-phenylalanine biotransformation, respectively. In that scenario, volumetric productivity and precursor yield were 39.6 mg2PE L-1h−1 and 0.69 g2PE per gram of L-phenylalanine added, respectively. From the PCA, it was identified that the reducing sugars content of the substrate, the air-filled porosity of the bed and the L-phenylalanine availability were the most critical parameters (associated with the substrates) influencing the microbial activity and 2-PE production. These results suggest that the desirable traits a solid media needs for promoting 2-PE production via SSF could be reached by using a combination of wastes in a synergistic approach.
KW - Aroma compounds
KW - Pichia kudriavzevii
KW - Rose-like compounds
KW - Solid-state fermentation
KW - Substrate screening
KW - Waste valorization
UR - http://www.scopus.com/inward/record.url?scp=85099193783&partnerID=8YFLogxK
U2 - 10.1016/j.wasman.2020.12.036
DO - 10.1016/j.wasman.2020.12.036
M3 - Artículo
C2 - 33445113
AN - SCOPUS:85099193783
VL - 121
SP - 403
EP - 411
JO - Waste Management
JF - Waste Management
SN - 0956-053X
ER -