TY - JOUR
T1 - Validation of three automated assays for total antioxidant capacity determination in canine serum samples
AU - Rubio, Camila Peres
AU - Hernández-Ruiz, Josefa
AU - Martinez-Subiela, Silvia
AU - Tvarijonaviciute, Asta
AU - Arnao, Marino Bañón
AU - Ceron, José Joaquín
PY - 2016/11/1
Y1 - 2016/11/1
N2 - © 2016, © 2016 The Author(s). We performed analytical validation of 3 automated assays of the total antioxidant capacity (TAC) in canine serum and evaluated their use in dogs with inflammatory bowel disease. The assays were based on the generation of a 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical cation (ABTS•+) in aqueous media, which produces a blue-green color. The antioxidants present in the sample remove the chromogen in proportion to their concentrations. The assays differed mainly in the way in which this radical was produced. All 3 assays produced acceptable results in the analytical validation. However, only 2 of the assays were capable of detecting significantly different TAC values in healthy and diseased animals.
AB - © 2016, © 2016 The Author(s). We performed analytical validation of 3 automated assays of the total antioxidant capacity (TAC) in canine serum and evaluated their use in dogs with inflammatory bowel disease. The assays were based on the generation of a 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical cation (ABTS•+) in aqueous media, which produces a blue-green color. The antioxidants present in the sample remove the chromogen in proportion to their concentrations. The assays differed mainly in the way in which this radical was produced. All 3 assays produced acceptable results in the analytical validation. However, only 2 of the assays were capable of detecting significantly different TAC values in healthy and diseased animals.
KW - ABTS
KW - inflammatory bowel disease
KW - metmyoglobin
KW - oxidative damage
KW - radical cation
U2 - 10.1177/1040638716664939
DO - 10.1177/1040638716664939
M3 - Article
VL - 28
SP - 693
EP - 698
JO - Journal of Veterinary Diagnostic Investigation
JF - Journal of Veterinary Diagnostic Investigation
SN - 1040-6387
IS - 6
ER -