Validation of three automated assays for total antioxidant capacity determination in canine serum samples

Camila Peres Rubio, Josefa Hernández-Ruiz, Silvia Martinez-Subiela, Asta Tvarijonaviciute, Marino Bañón Arnao, José Joaquín Ceron

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13 Citations (Scopus)

Abstract

© 2016, © 2016 The Author(s). We performed analytical validation of 3 automated assays of the total antioxidant capacity (TAC) in canine serum and evaluated their use in dogs with inflammatory bowel disease. The assays were based on the generation of a 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical cation (ABTS•+) in aqueous media, which produces a blue-green color. The antioxidants present in the sample remove the chromogen in proportion to their concentrations. The assays differed mainly in the way in which this radical was produced. All 3 assays produced acceptable results in the analytical validation. However, only 2 of the assays were capable of detecting significantly different TAC values in healthy and diseased animals.
Original languageEnglish
Pages (from-to)693-698
JournalJournal of Veterinary Diagnostic Investigation
Volume28
Issue number6
DOIs
Publication statusPublished - 1 Nov 2016

Keywords

  • ABTS
  • inflammatory bowel disease
  • metmyoglobin
  • oxidative damage
  • radical cation

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