Validation of an immunoturbidimetric method for determination of porcine serum C-reactive protein

Yolanda Saco, Lorenzo Fraile, Mercè Giménez, Francesca Canalias, Anna Bassols

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

Measurement of porcine C-reactive protein (CRP) in serum is an important tool for monitoring health and welfare in pigs. In this study, an immunoturbidimetric method from Olympus System Reagent (OSR 6147) used to measure human CRP in serum that employ a human traceable calibrator has been evaluated in porcine serum samples. Intra- and inter-assay imprecision were lower than that obtained with the porcine-specific commercially available ELISA. The expected difference in serum CRP between healthy and non-healthy pigs was detected. CRP values measured by the immunoturbidimetric method showed a good correlation with those obtained by ELISA, although differences in absolute CRP values were observed. When an in-house porcine standard was used a better agreement was obtained. In conclusion, the immunoturbidimetric method of Olympus can be used with porcine samples. The easier use of this method should facilitate the implementation of CRP serum determination for diagnostic and prognostic purposes in swine medicine. The results emphasize the need to establish species-specific standard and methods to decrease inter-laboratory discrepancies. © 2010 Elsevier Ltd.
Original languageEnglish
Pages (from-to)159-162
JournalResearch in Veterinary Science
Volume89
Issue number2
DOIs
Publication statusPublished - 1 Jan 2010

Keywords

  • CRP
  • Immunoturbidimetric method
  • Pig
  • Reference standard
  • Validation

Fingerprint

Dive into the research topics of 'Validation of an immunoturbidimetric method for determination of porcine serum C-reactive protein'. Together they form a unique fingerprint.

Cite this