Validated primer set that prevents nuclear DNA sequences of mitochondrial origin co-amplification: A revision based on the New Human Genome Reference Sequence (GRCh37)

Amanda Ramos, Cristina Santos, Elena Barbena, Ligia Mateiu, Luis Alvarez, Ramon Nogués, Maria Pilar Aluja

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21 Citations (Scopus)

Abstract

A new human genome reference sequence - GRCh37 - was recently generated and made available by the Genome Reference Consortium. Since the prior disposable human reference sequence - hg18 - was previously used for the mitochondrial DNA primer BLAST validation, a revision of those previously published primer pairs is required. Thus, the aim of this Short Communication is to perform an in silico BLAST test of the published disposable nine primer pairs using the new human reference sequence and to report the pertinent modifications. The new analysis showed that one of the tested primer pairs requires a revision. Therefore, a new validated primer pair, which specifically amplifies the mitochondrial region located between positions 6520 and 9184, is presented. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Original languageEnglish
Pages (from-to)782-783
JournalElectrophoresis
Volume32
DOIs
Publication statusPublished - 1 Mar 2011

Keywords

  • Mitochondrial DNA
  • Nuclear sequences of mitochondrial origin
  • Primer design

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