Vaccination with a genotype 1 modified live vaccine against porcine reproductive and respiratory syndrome virus significantly reduces viremia, viral shedding and transmission of the virus in a quasi-natural experimental model

Emanuela Pileri, Elisa Gibert, Ferran Soldevila, Ariadna García-Saenz, Joan Pujols, Ivan Diaz, Laila Darwich, Jordi Casal, Marga Martín, Enric Mateu

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31 Citations (Scopus)

Abstract

© 2014 Elsevier B.V. The present study assessed the efficacy of vaccination against genotype 1 porcine reproductive and respiratory syndrome virus (PRRSV) in terms of reduction of the transmission. Ninety-eight 3-week-old piglets were divided in two groups: V (n=40) and NV (n=58) that were housed separately. V animals were vaccinated with a commercial genotype 1 PRRSV vaccine while NV were kept as controls. On day 35 post-vaccination, 14 NV pigs were separated and inoculated intranasally with 2ml of a heterologous genotype 1 PRRSV isolate ("seeder" pigs, SP). The other V and NV animals were distributed in groups of 5 pigs each. Two days later, one SP was introduced into each pen to expose V and NV to PRRSV. Sentinel pigs were allocated in adjacent pens. Follow-up was of 21 days. All NV (30/30) became viremic after contact with SP while only 53% of V pigs were detected so (21/40, p<0.05). Vaccination shortened viremia (12.2±4 versus 3.7±3.4 days in NV and V pigs, respectively, p<0.01). The 50% survival time for becoming infected (Kaplan-Meier) for V was 21 days (CI<inf>95%</inf>=14.1-27.9) compared to 7 days (CI<inf>95%</inf>=5.2-8.7) for NV animals (p<0.01). These differences were reflected in the R value as well: 2.78 (CI<inf>95%</inf>=2.13-3.43) for NV and 0.53 (CI<inf>95%</inf>=0.19-0.76) for V pigs (p<0.05). All sentinel pigs (10/10) in pens adjacent to NV+SP pens got infected compared to 1/4 sentinel pigs allocated contiguous to a V+SP pen. These data show that vaccination of piglets significantly decrease parameters related to PRRSV transmission.
Original languageEnglish
Pages (from-to)7-16
JournalVeterinary Microbiology
Volume175
DOIs
Publication statusPublished - 1 Jan 2015

Keywords

  • Basic reproduction ratio
  • PRRS virus
  • Pig
  • Vaccine

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