In this study, central composite design and a response surface methodology are shown to be useful tools for medium optimization, in cultures of Pseudomonas aeruginosa 42A2 using an industrial oil byproduct, for polyhydroxyalkanoate (PHA) production. The optimum medium composition for PHA production includes: 80 g/L of the carbon source; 18.2 g/L of NaNO 3 and 3.3 g/L of K 2HPO 4/1.6 g/L KH 2PO 4 (R 2 = 0.989). The models were validated experimentally by cultivating Pseudomonas aeruginosa 42A2 in optimum media that yielded similar biomass (18.73 g/L) and PHA (4.63 g/L) concentrations to the predicted values. The optimized media showed an increase in biomass productivity from 0.06 to 0.39 g biomass/L h, and an increase in PHA productivity from 0.03 to 0.1 g PHA/L h. © 2011 AOCS.
|Journal||JAOCS, Journal of the American Oil Chemists' Society|
|Publication status||Published - 1 Jan 2012|
- Central rotatable composite design
- Medium optimization design
- Pseudomonas aeruginosa
- Response surface methodology
- Waste substrates