Utilization of agro-industrial residues for poly(3-hydroxyalkanoate) production by Pseudomonas aeruginosa 42A2 (NCIMB 40045): Optimization of culture medium

E. Rodríguez-Carmona, J. Bastida, A. Manresa

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5 Citations (Scopus)

Abstract

In this study, central composite design and a response surface methodology are shown to be useful tools for medium optimization, in cultures of Pseudomonas aeruginosa 42A2 using an industrial oil byproduct, for polyhydroxyalkanoate (PHA) production. The optimum medium composition for PHA production includes: 80 g/L of the carbon source; 18.2 g/L of NaNO 3 and 3.3 g/L of K 2HPO 4/1.6 g/L KH 2PO 4 (R 2 = 0.989). The models were validated experimentally by cultivating Pseudomonas aeruginosa 42A2 in optimum media that yielded similar biomass (18.73 g/L) and PHA (4.63 g/L) concentrations to the predicted values. The optimized media showed an increase in biomass productivity from 0.06 to 0.39 g biomass/L h, and an increase in PHA productivity from 0.03 to 0.1 g PHA/L h. © 2011 AOCS.
Original languageEnglish
Pages (from-to)111-122
JournalJAOCS, Journal of the American Oil Chemists' Society
Volume89
Issue number1
DOIs
Publication statusPublished - 1 Jan 2012

Keywords

  • Central rotatable composite design
  • Medium optimization design
  • PHA
  • Polyhydroxyalkanoates
  • Pseudomonas aeruginosa
  • Response surface methodology
  • Waste substrates

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