Uterine and placental specific localization of AQP2 and AQP8 is related with changes of serum progesterone levels in pregnant queens

Lluís Ferré-Dolcet, Marc Yeste, Meritxell Vendrell, Teresa Rigau, Joan Enric Rodríguez-Gil, Maria Montserrat Rivera del Alamo

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

Aquaporins play vital roles in reproductive physiology. This study evaluates the expression and localization dynamics of AQP1, AQP2, AQP3 and AQP8 in the endometrium and placental transference zone during pregnancy in queens by means of immunohistochemistry and Western blot. Animals were distributed into six groups: non-pregnant queens with low levels of serum progesterone (P-4), non-pregnant animals with high P-4 levels, and queens at 30, 40, 50 and 60 days of pregnancy. All AQPs were present in glandular and luminal epithelia and myometrium. AQP1 was also present in the endometrial endothelia. AQP2, AQP3 and AQP8 were found in trophoblast. In endometrial samples with P-4 above 2 ng/mL, AQP2 and AQP8 were distributed across plasma membrane and cytoplasm, whereas progesterone levels under 1 ng/mL kept both AQPs confined to the plasma membrane. Western blot showed no significant changes in AQPs expression among the stages. In conclusion, our results indicate that the distribution of AQP2 and AQP8 in the queen reproductive tract is related to P-4 levels. (C) 2019 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)149-157
Number of pages9
JournalTheriogenology
Volume142
DOIs
Publication statusPublished - 15 Jan 2020

Keywords

  • AQUAPORIN WATER CHANNELS
  • Aquaporins
  • CLONING
  • ENDOMETRIUM
  • ESTROUS-CYCLE
  • EXPRESSION
  • Endometrium
  • IMPLANTATION
  • MECHANISM
  • MEMBRANES
  • Placental transference zone
  • Pregnancy
  • Queen
  • REDISTRIBUTION
  • TRANSPORT

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