Usefulness of the SOS Chromotest in the study of medicinal plants as radioprotectors

Jorge Fuentes, Alena Alonso, Elizabeth Cuétara, Mariolys Vernhe, Nadine Alvarez, Angel Sánchez-Lamar, Montserrat Llagostera

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13 Citations (Scopus)

Abstract

Purpose: The aim of this work is to investigate the usefulness of a modified protocol of the SOS Chromotest to detect antigenotoxicity activities against γ-rays of plant extracts with proven antioxidant activity, and to elucidate the antigenotoxic mechanisms involved in radioprotection using this system. Materials and methods: The methodology developed was assayed with amifostine, the most studied radioprotector, and with Phyllanthus orbicularis HBK, Cymbopogon citratus (DC) Stapf and Pinus caribaea Morelet extracts, using pre- and post-treatment procedures. Results: The P. caribaea and C. citratus extracts were antigenotoxic against γ-rays when the cells were pre-treated with both extracts, suggesting a possible antigenotoxic action through a free radical scavenging mechanisms. Amifostine and the P. orbicularis extract were also antigenotoxic under pre- and post-treatment conditions, indicating that several antimutagenic components of this plant extract may also operate by some intracellular mechanism, unlike its antioxidant activity. Conclusions: The results have demonstrated the usefulness of the modified SOS Chromotest assay in the screening of phytochemical radioprotectors as well as in the study of their antimutagenic mechanisms. © 2006 Taylor & Francis.
Original languageEnglish
Pages (from-to)323-329
JournalInternational Journal of Radiation Biology
Volume82
Issue number5
DOIs
Publication statusPublished - 1 May 2006

Keywords

  • γ-rays
  • Amifostine
  • Escherichia coli
  • Medicinal plants
  • Radioprotectors
  • SOS Chromotest

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