TY - JOUR
T1 - Usefulness of Oral Fluid for Measurement of Methylone and Its Metabolites:
T2 - Correlation with Plasma Drug Concentrations and the Effect of Oral Fluid pH
AU - Sprega, Giorgia
AU - Di Giorgi, Alessandro
AU - Poyatos, Lourdes
AU - Papaseit, Esther
AU - Pérez-Mañá, Clara
AU - Tini, Anastasio
AU - Pichini, Simona
AU - Busardò, Francesco Paolo
AU - Lo Faro, Alfredo Fabrizio
AU - Farré, Magí
N1 - Publisher Copyright:
© 2023 by the authors.
PY - 2023/3/24
Y1 - 2023/3/24
N2 - The aim of this study was to investigate methylone and its metabolites concentration in oral fluid following controlled increasing doses, focusing on the effect of oral fluid pH. Samples were obtained from a clinical trial where twelve healthy volunteers participated after ingestion of 50, 100, 150 and 200 mg of methylone. Concentration of methylone and its metabolites 4-hydroxy-3-methoxy-N-methylcathinone (HMMC) and 3,4-methylenedioxycathinone in oral fluid were measured using liquid chromatography–tandem mass spectrometry (LC–MS/MS). Pharmacokinetic parameters were estimated, and the oral fluid-to-plasma ratio (OF/P) at each time interval was calculated and correlated with the oral fluid pH using data from our previous study in plasma. Methylone was detected at all time intervals after each dose; MDC and HMMC were not detectable after the lowest dose. Oral fluid concentrations of methylone ranged between 88.3–503.8, 85.5–5002.3, 182.8–13,201.8 and 214.6–22,684.6 ng/mL following 50, 100, 150 and 200 mg doses, respectively, peaked between 1.5 and 2.0 h, and were followed by a progressive decrease. Oral fluid pH was demonstrated to be affected by methylone administration. Oral fluid is a valid alternative to plasma for methylone determination for clinical and toxicological studies, allowing for a simple, easy and non-invasive sample collection.
AB - The aim of this study was to investigate methylone and its metabolites concentration in oral fluid following controlled increasing doses, focusing on the effect of oral fluid pH. Samples were obtained from a clinical trial where twelve healthy volunteers participated after ingestion of 50, 100, 150 and 200 mg of methylone. Concentration of methylone and its metabolites 4-hydroxy-3-methoxy-N-methylcathinone (HMMC) and 3,4-methylenedioxycathinone in oral fluid were measured using liquid chromatography–tandem mass spectrometry (LC–MS/MS). Pharmacokinetic parameters were estimated, and the oral fluid-to-plasma ratio (OF/P) at each time interval was calculated and correlated with the oral fluid pH using data from our previous study in plasma. Methylone was detected at all time intervals after each dose; MDC and HMMC were not detectable after the lowest dose. Oral fluid concentrations of methylone ranged between 88.3–503.8, 85.5–5002.3, 182.8–13,201.8 and 214.6–22,684.6 ng/mL following 50, 100, 150 and 200 mg doses, respectively, peaked between 1.5 and 2.0 h, and were followed by a progressive decrease. Oral fluid pH was demonstrated to be affected by methylone administration. Oral fluid is a valid alternative to plasma for methylone determination for clinical and toxicological studies, allowing for a simple, easy and non-invasive sample collection.
KW - humans
KW - LC–MS/MS
KW - methylone
KW - oral fluid
KW - pharmacokinetics
KW - Humans
KW - LC–MS/MS
KW - Methylone
KW - Oral fluid
KW - Pharmacokinetics
KW - Humans
KW - LC–MS/MS
KW - Methylone
KW - Oral fluid
KW - Pharmacokinetics
UR - http://www.scopus.com/inward/record.url?scp=85153779005&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/8910561d-fd8a-3f24-9314-e22186938d00/
U2 - 10.3390/metabo13040468
DO - 10.3390/metabo13040468
M3 - Article
C2 - 37110127
AN - SCOPUS:85153779005
SN - 2218-1989
VL - 13
JO - Metabolites
JF - Metabolites
IS - 4
M1 - 468
ER -