Use of the TRP1 auxotrophic marker for gene disruption and phenotypic analysis in yeast: A note of warning

Asier González, Carolina Larroy, Josep A. Biosca, Joaquín Ariño

Research output: Contribution to journalArticleResearchpeer-review

14 Citations (Scopus)

Abstract

The TRP1 marker has been commonly used for gene disruption experiments and subsequent phenotypic analysis. However, introduction of the TRP1 gene into a trp1 strain markedly affects growth under many conditions used for phenotypic profiling. Therefore, its use in the past should be revisited and utilization of this marker should be avoided in future analyses. © 2007 Federation of European Microbiological Societies.
Original languageEnglish
Pages (from-to)2-5
JournalFEMS Yeast Research
Volume8
Issue number1
DOIs
Publication statusPublished - 1 Feb 2008

Keywords

  • Auxotrophic marker
  • Phenotypic analysis
  • TRP1 gene

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