Use of the TRP1 auxotrophic marker for gene disruption and phenotypic analysis in yeast: A note of warning

Asier González; Carolina Larroy; Josep A. Biosca; Joaquín Ariño

doi:10.1111/j.1567-1364.2007.00315.x

Use of the TRP1 auxotrophic marker for gene disruption and phenotypic analysis in yeast: A note of warning

Asier González, Carolina Larroy, Josep A. Biosca, Joaquín Ariño

Department of Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › Research › peer-review

15 Citations (Scopus)

Abstract

The TRP1 marker has been commonly used for gene disruption experiments and subsequent phenotypic analysis. However, introduction of the TRP1 gene into a trp1 strain markedly affects growth under many conditions used for phenotypic profiling. Therefore, its use in the past should be revisited and utilization of this marker should be avoided in future analyses. © 2007 Federation of European Microbiological Societies.

Original language	English
Pages (from-to)	2-5
Journal	FEMS Yeast Research
Volume	8
Issue number	1
DOIs	https://doi.org/10.1111/j.1567-1364.2007.00315.x
Publication status	Published - 1 Feb 2008

Keywords

Auxotrophic marker
Phenotypic analysis
TRP1 gene

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10.1111/j.1567-1364.2007.00315.x

Cite this

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abstract = "The TRP1 marker has been commonly used for gene disruption experiments and subsequent phenotypic analysis. However, introduction of the TRP1 gene into a trp1 strain markedly affects growth under many conditions used for phenotypic profiling. Therefore, its use in the past should be revisited and utilization of this marker should be avoided in future analyses. {\textcopyright} 2007 Federation of European Microbiological Societies.",

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AU - Biosca, Josep A.

AU - Ariño, Joaquín

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AB - The TRP1 marker has been commonly used for gene disruption experiments and subsequent phenotypic analysis. However, introduction of the TRP1 gene into a trp1 strain markedly affects growth under many conditions used for phenotypic profiling. Therefore, its use in the past should be revisited and utilization of this marker should be avoided in future analyses. © 2007 Federation of European Microbiological Societies.

KW - Auxotrophic marker

KW - Phenotypic analysis

KW - TRP1 gene

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DO - 10.1111/j.1567-1364.2007.00315.x

M3 - Article

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JO - FEMS Yeast Research

JF - FEMS Yeast Research

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Use of the TRP1 auxotrophic marker for gene disruption and phenotypic analysis in yeast: A note of warning

Abstract

Keywords

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