Use of Immobilized Microbial Membrane Fragments To Remove Oxygen and Favor the Acetone‐Butanol Fermentation

Francesc Godia; Howard I. Adler; Charles D. Scott; Brian H. Davison

doi:10.1021/bp00003a009

Use of Immobilized Microbial Membrane Fragments To Remove Oxygen and Favor the Acetone‐Butanol Fermentation

Francesc Godia, Howard I. Adler, Charles D. Scott, Brian H. Davison

Department of Chemistry

Research output: Contribution to journal › Article › Research › peer-review

4 Citations (Scopus)

Abstract

Oxygen‐reducing membrane fragments obtained from Escherichia coli were used with Clostridium acetobutylicum (C. acetobylicum) to provide an oxygen‐free microenvironment for the conversion of glucose to acetone, butanol, and ethanol (ABE). The batch fermentation of suspended C. acetobutylicum NRRL‐B‐643 and its ability to produce solvents in the presence of membranes as the oxygen‐elimination agent are described and compared with the conventional sparging technique used to maintain anaerobiosis. The use of membrane fragments to remove oxygen for fermentation by C. acetobutylicum was successful and gave slightly improved results over the use of sparging with regard to lag, biomass, and solvent production (e.g., final butanol concentration of 3.25 and 2.7 g/L, respectively). Solvent production is also reported for a continuous columnar reactor with coimmobilized cells and membranes in K‐carrageenan gel beads and air‐saturated liquid feed. Copyright © 1990 American Institute of Chemical Engineers (AIChE)

Original language	English
Pages (from-to)	210-213
Journal	Biotechnology Progress
Volume	6
Issue number	3
DOIs	https://doi.org/10.1021/bp00003a009
Publication status	Published - 1 Jan 1990

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title = "Use of Immobilized Microbial Membrane Fragments To Remove Oxygen and Favor the Acetone‐Butanol Fermentation",

abstract = "Oxygen‐reducing membrane fragments obtained from Escherichia coli were used with Clostridium acetobutylicum (C. acetobylicum) to provide an oxygen‐free microenvironment for the conversion of glucose to acetone, butanol, and ethanol (ABE). The batch fermentation of suspended C. acetobutylicum NRRL‐B‐643 and its ability to produce solvents in the presence of membranes as the oxygen‐elimination agent are described and compared with the conventional sparging technique used to maintain anaerobiosis. The use of membrane fragments to remove oxygen for fermentation by C. acetobutylicum was successful and gave slightly improved results over the use of sparging with regard to lag, biomass, and solvent production (e.g., final butanol concentration of 3.25 and 2.7 g/L, respectively). Solvent production is also reported for a continuous columnar reactor with coimmobilized cells and membranes in K‐carrageenan gel beads and air‐saturated liquid feed. Copyright {\textcopyright} 1990 American Institute of Chemical Engineers (AIChE)",

author = "Francesc Godia and Adler, {Howard I.} and Scott, {Charles D.} and Davison, {Brian H.}",

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T1 - Use of Immobilized Microbial Membrane Fragments To Remove Oxygen and Favor the Acetone‐Butanol Fermentation

AU - Godia, Francesc

AU - Adler, Howard I.

AU - Scott, Charles D.

AU - Davison, Brian H.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - Oxygen‐reducing membrane fragments obtained from Escherichia coli were used with Clostridium acetobutylicum (C. acetobylicum) to provide an oxygen‐free microenvironment for the conversion of glucose to acetone, butanol, and ethanol (ABE). The batch fermentation of suspended C. acetobutylicum NRRL‐B‐643 and its ability to produce solvents in the presence of membranes as the oxygen‐elimination agent are described and compared with the conventional sparging technique used to maintain anaerobiosis. The use of membrane fragments to remove oxygen for fermentation by C. acetobutylicum was successful and gave slightly improved results over the use of sparging with regard to lag, biomass, and solvent production (e.g., final butanol concentration of 3.25 and 2.7 g/L, respectively). Solvent production is also reported for a continuous columnar reactor with coimmobilized cells and membranes in K‐carrageenan gel beads and air‐saturated liquid feed. Copyright © 1990 American Institute of Chemical Engineers (AIChE)

AB - Oxygen‐reducing membrane fragments obtained from Escherichia coli were used with Clostridium acetobutylicum (C. acetobylicum) to provide an oxygen‐free microenvironment for the conversion of glucose to acetone, butanol, and ethanol (ABE). The batch fermentation of suspended C. acetobutylicum NRRL‐B‐643 and its ability to produce solvents in the presence of membranes as the oxygen‐elimination agent are described and compared with the conventional sparging technique used to maintain anaerobiosis. The use of membrane fragments to remove oxygen for fermentation by C. acetobutylicum was successful and gave slightly improved results over the use of sparging with regard to lag, biomass, and solvent production (e.g., final butanol concentration of 3.25 and 2.7 g/L, respectively). Solvent production is also reported for a continuous columnar reactor with coimmobilized cells and membranes in K‐carrageenan gel beads and air‐saturated liquid feed. Copyright © 1990 American Institute of Chemical Engineers (AIChE)

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DO - 10.1021/bp00003a009

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JO - Biotechnology Progress

JF - Biotechnology Progress

IS - 3

ER -

Use of Immobilized Microbial Membrane Fragments To Remove Oxygen and Favor the Acetone‐Butanol Fermentation

Abstract

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