Use of hypometabolic TRIS extenders and high cooling rate refrigeration for cryopreservation of stallion sperm: Presence and sensitivity of 5' AMP-activated protein kinase (AMPK)

Alex Córdova, Pablo Strobel, Andrés Vallejo, Pamela Valenzuela, Omar Ulloa, Rafael A. Burgos, Bruno Menarim, Joan Enric Rodríguez-Gil, Marcelo Ratto, Alfredo Ramírez-Reveco

Research output: Contribution to journalArticleResearchpeer-review

30 Citations (Scopus)

Abstract

© 2014 Elsevier Inc. This study evaluated the effect of the use of hypometabolic TRIS extenders in the presence or the absence of AMPK activators as well as the utilization of high cooling rates in the refrigeration step on the freezability of stallion sperm. Twelve ejaculates were cryopreserved using Botucrio® as a control extender and a basic TRIS extender (HM-0) separately supplemented with 10. mM metformin, 2. mM 5-aminoimidazole-4-carboxamide-1-β- d-ribofuranoside (AICAR), 2. mM Adenosine monophosphate (AMP), 40. μM compound C AMPK inhibitor or 2. mM AMP. +. 40. μM compound C. Our results showed that the utilization of a hypometabolic TRIS extender supplemented or not with AMP or metformin significantly improves stallion sperm freezability when compared with a commercial extender. Additionally, high cooling rates do not affect stallion sperm quality after cooling and post-thawing. Finally, stallion spermatozoa present several putative AMPK sperm isoforms that do not seem to respond to classical activators, but do respond to the Compound C inhibitor.
Original languageEnglish
Pages (from-to)473-481
JournalCryobiology
Volume69
DOIs
Publication statusPublished - 1 Jan 2014

Keywords

  • AMPK
  • Cryopreservation
  • Equine
  • Hypometabolism
  • Spermatozoa

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